{"doi":"10.1093/nar/13.17.6035","title":"Cloning of the chick hsp 90 cDNA in expression vector","abstract":null,"journal":"Nucleic Acids Research","year":1985,"id":662840,"datarank":0.6064576901751826,"base_score":4.04305126783455,"endowment":4.04305126783455,"self_citation_contribution":0.6064576901751826,"citation_network_contribution":0.0,"self_endowment_contribution":0.6064576901751826,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":56,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":70626,"name":"Nadine Binart","orcid":"0000-0002-5606-3866","position":1,"is_corresponding":false},{"id":1730501,"name":"James R. Feramisco","orcid":null,"position":2,"is_corresponding":false},{"id":171463,"name":"David M. Helfman","orcid":null,"position":3,"is_corresponding":false},{"id":1730500,"name":"Maria Grazia Catelli","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cloning of the chick hsp 90 cDNA in expression vector","abstract":"A cDNA clone for the 90kDa heat-shock protein, which we have recently identified as a component of steroid hormone receptors in their heteromeric 8S form, was isolated by direct immunological screening of a chicken smooth muscle cDNA expression library, prepared in the expression plasmids pUC8 and pUC9. Using polyclonal and monoclonal antibodies against the 90kDa protein a colony was identified that reacted with both antibodies. Plasmid 9.11 (p9.11, approximately 1100 base pair insert) was found to hybrid-select mRNA for the 90kDa heat-shock protein. Northern blot analysis revealed that RNA isolated from various chicken tissues contain a single transcript of approximately 3 Kb hybridizing to a [32P]labelled cDNA probe made from p9.11. Heat-shock treatment of chick embryonic fibroblasts resulted in increased steady-state levels of a 3 Kb transcript in both poly A+ and poly A- RNA fractions. Southern blot analysis of chicken genomic DNA indicated that the cDNA hybridizes to a single copy sequence. Sequence data show that the p9.11 cDNA displays a high degree of homology with the 5' portion of yeast heat shock protein 90 cDNA.","is_dataset_classified":null,"base_score":4.04305126783455,"endowment":4.04305126783455,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"3900927","pmcid":"PMC321936","openalex_id":"https://openalex.org/W2116623627","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA 29569","title":null}],"total_grants":1,"fwci":3.6553,"citation_percentile":0.9403087,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2017,"count":1},{"year":2020,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/13/17/6035/3260724/13-17-6035.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/13/17/6035/3260724/13-17-6035.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/13/17/6035/3260724/13-17-6035.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/13.17.6035","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/3900927","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/321936","host_type":"repository"},{"url":"http://nar.oxfordjournals.org/cgi/content/short/13/17/6035","host_type":"repository"}],"fields_of_study":["Heat shock proteins research","Viral Infectious Diseases and Gene Expression in Insects","thermodynamics and calorimetric analyses"],"mesh_terms":["Amino Acid Sequence","Animals","Base Composition","Base Sequence","Chickens","Cloning, Molecular","DNA","Genetic Vectors","Heat-Shock Proteins","Nucleic Acid Hybridization","Rabbits","Reticulocytes","RNA, Messenger","Saccharomyces cerevisiae","Species Specificity","Protein Biosynthesis"],"keywords":["Complementary DNA","Biology","Molecular biology","cDNA library","Northern blot","Plasmid","RNA","Polyclonal antibodies","Heat shock protein","genomic DNA","Southern blot","Messenger RNA","Gene","Antibody","Biochemistry","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T17:44:31.723329Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}