{"doi":"10.1093/jxb/erv101","title":"Sucrose phosphate synthase and sucrose phosphate phosphatase interact<i>in planta</i>and promote plant growth and biomass accumulation","abstract":null,"journal":"Journal of Experimental Botany","year":2015,"id":598881,"datarank":0.7181237614173069,"base_score":4.787491742782046,"endowment":4.787491742782046,"self_citation_contribution":0.7181237614173069,"citation_network_contribution":0.0,"self_endowment_contribution":0.7181237614173069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":119,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":790096,"name":"Ji-Young Park","orcid":"0000-0001-9672-8685","position":1,"is_corresponding":false},{"id":1534826,"name":"Faride Unda","orcid":null,"position":2,"is_corresponding":false},{"id":1135861,"name":"Shawn D. Mansfield","orcid":"0000-0002-0175-554X","position":3,"is_corresponding":false},{"id":1534824,"name":"Victoria J. Maloney","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Sucrose phosphate synthase and sucrose phosphate phosphatase interact<i>in planta</i>and promote plant growth and biomass accumulation","abstract":"Bioinformatic analysis indicates that sucrose phosphate synthase (SPS) contains a putative C-terminal sucrose phosphate phosphatase (SPP)-like domain that may facilitates the binding of SPP. If an SPS-SPP enzyme complex exists, it may provide sucrose biosynthesis with an additional level of regulation, forming a direct metabolic channel for sucrose-6-phosphate between these two enzymes. Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins. In addition, in planta bioluminescence resonance energy transfer (BRET) was observed in Arabidopsis seedlings, providing physical evidence for a protein interaction in live cells and in real time. Finally, bimolecular fluorescence complementation (BiFC) was employed in an attempt to detect SPS-SPP interactions visually. The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch. Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.","is_dataset_classified":null,"base_score":4.787491742782046,"endowment":4.787491742782046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25873678","pmcid":"PMC4493782","openalex_id":"https://openalex.org/W2106517787","authors":[],"funders":[{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"unidentified","title":"unidentified"}],"total_grants":1,"fwci":9.6056,"citation_percentile":0.97566171,"influential_citations":0,"citation_trend":[{"year":2015,"count":2},{"year":2016,"count":6},{"year":2017,"count":2},{"year":2018,"count":10},{"year":2019,"count":8},{"year":2020,"count":13},{"year":2021,"count":30},{"year":2022,"count":14},{"year":2023,"count":9},{"year":2024,"count":11},{"year":2025,"count":6},{"year":2026,"count":8}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://academic.oup.com/jxb/article-pdf/66/14/4383/9570410/erv101.pdf","host_type":"journal"},{"url":"https://academic.oup.com/jxb/article-pdf/66/14/4383/9570410/erv101.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/jxb/erv101","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25873678","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4493782","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4493782","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4493782?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1093/jxb/erv101","host_type":""},{"url":"https://dx.doi.org/10.1093/jxb/erv101","host_type":""}],"fields_of_study":["Plant nutrient uptake and metabolism","Photosynthetic Processes and Mechanisms","Polysaccharides and Plant Cell Walls","0301 basic medicine","0303 health sciences","03 medical and health sciences","Arabidopsis","Biomass","Glucosyltransferases","Phosphoprotein Phosphatases","Plants","Protein Binding"],"mesh_terms":["Glucosyltransferases","Phosphoprotein Phosphatases","Plants","Protein Binding","Arabidopsis","Biomass"],"keywords":["Sucrose-phosphate synthase","Bimolecular fluorescence complementation","Arabidopsis","Biochemistry","Sucrose","Sugar phosphates","Complementation","Phosphate","Phosphatase","Sucrose synthase","Enzyme","Arabidopsis thaliana","Biology","Yeast","Protein-fragment complementation assay","Chemistry","Invertase","Gene","Phenotype","Mutant","Biomass","Cellulose","Poplar","sucrose phosphate synthase","Protein–protein Interaction","Bret","Bifc","Yeast Two-hybrid.","Sucrose Phosphate Phosphatase","Glucosyltransferases","Phosphoprotein Phosphatases","Plants","Research Paper","Protein Binding"],"sdg_mappings":[{"sdg_number":7,"sdg_label":"7. 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