{"doi":"10.1093/jxb/eri244","title":"Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula","abstract":null,"journal":"Journal of Experimental Botany","year":2005,"id":589011,"datarank":1.0844562224986034,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.6427903756236373,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.6427903756236373,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":13,"citers_with_citation_signal":12,"citers_with_endowment":12,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1506977,"name":"Andreas Niebel","orcid":null,"position":1,"is_corresponding":false},{"id":1506978,"name":"Aurélien Boisson-Dernier","orcid":null,"position":2,"is_corresponding":false},{"id":1506979,"name":"David G. Barker","orcid":null,"position":3,"is_corresponding":false},{"id":1506980,"name":"Fernanda de Carvalho-Niebel","orcid":null,"position":4,"is_corresponding":false},{"id":1506976,"name":"Laurent Sauviac","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula","abstract":"This article describes an efficient procedure to study Nod factor-induced gene expression in root hairs of the model legume Medicago truncatula. By developing an improved method of fracturing frozen root hairs, it has been possible to obtain a highly purified root hair fraction from M. truncatula seedlings yielding sufficient RNA for real-time quantitative RT-PCR expression analysis. After Nod factor treatment it was possible to detect up to 100-fold increases of MtENOD11 and pMtENOD11-gus transcript levels in root hair RNA. This corresponds to 5-7-fold higher induction levels than for entire root tissue preparations. Furthermore, the use of these enriched RNA samples has revealed for the first time a very significant induction (30-fold) of the MtENOD40 gene in root hairs in response to Nod factors. It is concluded that the rapid and convenient procedure described here will be particularly useful for detecting tissue-specific low-level gene expression in root hairs responding to Rhizobium Nod factors or other exogenous signals.","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16043451","pmcid":null,"openalex_id":"https://openalex.org/W2169033980","authors":[],"funders":[],"total_grants":0,"fwci":2.3148,"citation_percentile":0.89398744,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2022,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/jxb/article-pdf/56/419/2507/1421995/eri244.pdf","host_type":"journal"},{"url":"https://academic.oup.com/jxb/article-pdf/56/419/2507/1421995/eri244.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/jxb/article-pdf/56/419/2507/1421995/eri244.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/jxb/eri244","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16043451","host_type":"repository"},{"url":"https://cnrs.hal.science/hal-03082465","host_type":"repository"}],"fields_of_study":["Legume Nitrogen Fixing Symbiosis","Plant nutrient uptake and metabolism","Agronomic Practices and Intercropping Systems"],"mesh_terms":["Base Sequence","Membrane Proteins","Plant Proteins","Transcription, Genetic","Polymerase Chain Reaction","DNA Primers","Gene Expression Regulation, Plant","Plant Roots","Reverse Transcriptase Polymerase Chain Reaction","Medicago truncatula"],"keywords":["Medicago truncatula","Nod factor","Root hair","Medicago","Nod","Biology","Gene expression","Legume","RNA","Gene","Botany","Root nodule","Cell biology","Molecular biology","Symbiosis","Genetics","Bacteria"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T11:44:33.347167Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}