{"doi":"10.1093/jmcb/mjae047","title":"Cohesin ring gates are specialized for meiotic cell division","abstract":"Cohesin is a ring complex closed with structural maintenance of chromosome 1 (SMC-1), SMC-3, and a kleisin subunit, mediating sister chromatid cohesion in mitosis and meiosis. Kleisin N- and C-terminal domains interact with SMC-3 and SMC-1, forming two distinct cohesin gates. Whether these gates are specialized for mitosis and meiosis remains elusive. Here, we create Caenorhabditis elegans mutants that express chimeric proteins swapping N- and C-terminal domains between different kleisins to investigate how these gates are specialized for different cell division programs. Replacing the meiotic REC-8 N-terminus with that of a cell division-unrelated kleisin COH-1 or the mitotic kleisin sister chromatid cohesion protein 1 (SCC-1) disrupts inter-sister chromatid cohesion and causes severe meiotic defects. Swapping the REC-8 C-terminus with that of COH-1 or SCC-1 largely retains the meiotic functions of REC-8 but causes age-related chromosome abnormalities. A specialized C-terminus is also required for the functions of SCC-1. Furthermore, point mutations in the REC-8 C-terminus cause severe meiotic defects without impairing the SMC-1-kleisin interaction, suggesting an integrated SMC-1-kleisin gate. These findings suggest the requirements for specialized cohesin gates in different biological processes.","journal":"Journal of Molecular Cell Biology","year":2024,"id":469496,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9536,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1261369,"name":"Bohan Liu","orcid":"0000-0002-8318-9667","position":1,"is_corresponding":false},{"id":1094465,"name":"Ruirui Zhang","orcid":"0000-0002-3328-1318","position":2,"is_corresponding":false},{"id":1303167,"name":"Zixuan Zhu","orcid":"0000-0002-4899-4556","position":3,"is_corresponding":false},{"id":310323,"name":"Li Zhao","orcid":"0000-0003-3329-7376","position":4,"is_corresponding":false},{"id":1303579,"name":"Ruijie Jiang","orcid":null,"position":5,"is_corresponding":false},{"id":1303168,"name":"Yinghao Wang","orcid":"0000-0002-0045-3531","position":6,"is_corresponding":false},{"id":724639,"name":"Feifei Qi","orcid":"0000-0003-0150-1512","position":7,"is_corresponding":false},{"id":310315,"name":"Ruoxi Wang","orcid":"0000-0002-8417-1987","position":8,"is_corresponding":false},{"id":850829,"name":"Huijie Zhao","orcid":"0000-0002-8595-8159","position":9,"is_corresponding":false},{"id":310325,"name":"Jun Zhou","orcid":"0000-0003-3131-7804","position":10,"is_corresponding":false},{"id":310326,"name":"Jinmin Gao","orcid":"0000-0002-5894-6515","position":11,"is_corresponding":false},{"id":310317,"name":"Yuanyuan Liu","orcid":"0000-0002-4404-7041","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-19T02:05:32.241298Z","pmid":"39401990","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}