{"doi":"10.1093/jmcb/mjab058","title":"SKAP interacts with Aurora B to guide end-on capture of spindle microtubules via phase separation","abstract":"Chromosome segregation in mitosis is orchestrated by the dynamic interactions between the kinetochore and spindle microtubules. Our recent studies show that mitotic motor CENP-E cooperates with SKAP and forms a link between kinetochore core MIS13 complex and spindle microtubule plus-ends to achieve accurate chromosome alignment in mitosis. However, it remains elusive how SKAP regulates kinetochore attachment from lateral association to end-on attachment during metaphase alignment. Here, we identify a novel interaction between Aurora B and SKAP that orchestrates accurate interaction between the kinetochore and dynamic spindle microtubules. Interestingly, SKAP spontaneously phase-separates in vitro via weak, multivalent interactions into droplets with fast internal dynamics. SKAP and Aurora B form heterogeneous coacervates in vitro, which recapitulate the dynamics and behavior of SKAP comets in vivo. Importantly, SKAP interaction with Aurora B via phase separation is essential for accurate chromosome segregation and alignment. Based on those findings, we reason that SKAP-Aurora B interaction via phase separation constitutes a dynamic pool of Aurora B activity during the lateral to end-on conversion of kinetochore-microtubule attachments to achieve faithful cell division.","journal":"Journal of Molecular Cell Biology","year":2021,"id":191263,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":686498,"name":"Fengrui Yang","orcid":"0009-0006-7132-4792","position":1,"is_corresponding":false},{"id":202935,"name":"Wenwen Wang","orcid":"0000-0002-1545-4369","position":2,"is_corresponding":false},{"id":756834,"name":"Najdat Zohbi","orcid":null,"position":3,"is_corresponding":false},{"id":756304,"name":"Xiwei Wang","orcid":"0000-0002-3970-1336","position":4,"is_corresponding":false},{"id":692838,"name":"Dongmei Wang","orcid":"0000-0003-2632-6562","position":5,"is_corresponding":false},{"id":625482,"name":"Xiaoxuan Zhuang","orcid":"0000-0003-4004-5329","position":6,"is_corresponding":false},{"id":293201,"name":"Zhen Dou","orcid":"0000-0003-3303-9393","position":7,"is_corresponding":false},{"id":311110,"name":"Dan Liu","orcid":"0000-0002-2198-3910","position":8,"is_corresponding":false},{"id":756305,"name":"Xiaoyu Song","orcid":"0000-0002-7598-0459","position":9,"is_corresponding":false},{"id":756835,"name":"Hadiyah-Nicole Green","orcid":null,"position":10,"is_corresponding":false},{"id":58796,"name":"Xing Liu","orcid":"0000-0002-7480-5085","position":11,"is_corresponding":false},{"id":293205,"name":"Xuebiao Yao","orcid":"0000-0001-8982-5911","position":12,"is_corresponding":false},{"id":756833,"name":"Manjuan Zhang","orcid":null,"position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-18T23:49:31.121780Z","pmid":"34554241","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}