{"doi":"10.1093/jimmun/vkaf283.2005","title":"Oral antigen administration prevents allergic sensitization in distant lymph nodes by preventing development of antigen-specific T follicular helper cells 4305","abstract":"Abstract Description Early oral route antigen exposure decreases the incidence of food allergy while environmental exposures through non-oral routes, such as the skin or airways, increase the risk. The goal of this study was to investigate the immunologic mechanisms of how oral allergen consumption protects from sensitization to the same allergens through environmental exposure routes. Naive mice were orally administered ovalbumin (OVA) in drinking water. Mice were subsequently exposed intranasally (i.n.) or epicutaneously (e.c.) to OVA plus peanut flour, as an adjuvant. OVA-specific CD4+ T cells from draining lymph nodes (dLNs) were assessed by MHC-II tetramer staining and flow cytometry. Following i.n. or e.c. exposure to OVA plus peanut flour, mice developed OVA-IgE and OVA-IgG1 and showed symptoms of acute anaphylaxis upon OVA challenge. Oral OVA feeding prior to i.n. or e.c. antigen exposure dramatically reduced OVA-IgE titers and protected mice from anaphylaxis. Protection was OVA-specific, as OVA-fed mice still developed peanut-IgE and anaphylaxis upon i.p. peanut challenge. Total OVA-specific CD4+ T and CD4+Foxp3+ T regulatory (Treg) cell numbers in dLNs were similar between control and OVA-fed mice. However, the majority of OVA-specific CD4+ T cells in control dLNs were T follicular helper (Tfh) cells, which were nearly abolished in OVA-fed mice. Oral route antigen exposure suppresses development of antigen-specific Tfh cells in remote dLNs and prevents systemic allergic sensitization. Funding Sources Supported by NIH R37 AI71106 and Mayo Clinic Graduate School of Biomedical Sciences. Topic Categories Immediate Hypersensitivity, Asthma, and Allergic Responses (HYP)","journal":"The Journal of Immunology","year":2025,"id":582328,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9562,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":483972,"name":"Koji Iijima","orcid":null,"position":1,"is_corresponding":false},{"id":1314311,"name":"Mayumi Matsunaga","orcid":"0000-0001-5152-6617","position":2,"is_corresponding":false},{"id":483119,"name":"Takao Kobayashi","orcid":"0000-0002-5255-9573","position":3,"is_corresponding":false},{"id":1494409,"name":"Jared Brooksby","orcid":null,"position":4,"is_corresponding":false},{"id":1306832,"name":"Min-Jhen Jheng","orcid":null,"position":5,"is_corresponding":false},{"id":344287,"name":"Hirohito Kita","orcid":"0000-0002-6854-2936","position":6,"is_corresponding":false},{"id":849936,"name":"Mia Y. Masuda","orcid":"0000-0001-7085-0156","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:58:55.657290Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}