{"doi":"10.1093/jimmun/vkaf223","title":"Dynamic control of autoreactive B cells from spontaneous germinal centers","abstract":"Systemic lupus erythematosus is characterized by activation of many self-reactive B cell clones that produce autoantibodies. This can be modeled using mixed bone marrow chimeras, where autoreactive 564Igi B cells initiate autoimmunity that spreads to wild-type (WT) B cells. The mechanisms controlling the inclusion of new B cell clones into spontaneous germinal centers (GCs) remain unclear. Using CRISPR-Cas9, we generated 2 autoreactive B cell receptor knock-in strains, M05 and G55, based on B cell receptors from WT GC B cells in WT:564Igi chimeras. M05 and G55 mice lacked spontaneous GCs and overt autoantibody production, with receptor editing (λ light chain expression) contributing to tolerance. However, autoreactivity was not purged from the B cell compartment since presence of 564Igi clone allowed M05 and G55 B cells to join GCs and produce autoantibodies. These findings reveal that GCs can override peripheral tolerance, recruiting previously silent autoreactive clones and facilitating diversification of autoantibodies.","journal":"The Journal of Immunology","year":2025,"id":547334,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9563,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1440185,"name":"Alexia Correia Ferreira","orcid":null,"position":1,"is_corresponding":false},{"id":1440186,"name":"Selma Mouftakir","orcid":null,"position":2,"is_corresponding":false},{"id":498104,"name":"Carlos Castrillón","orcid":"0000-0003-2909-7371","position":3,"is_corresponding":false},{"id":1436683,"name":"Lisa Madungwe","orcid":null,"position":4,"is_corresponding":false},{"id":455443,"name":"Usha Nair","orcid":"0000-0002-1927-6889","position":5,"is_corresponding":false},{"id":663809,"name":"Facundo D. Batista","orcid":"0000-0002-1130-9463","position":6,"is_corresponding":false},{"id":16703,"name":"Michael C. Carroll","orcid":"0000-0002-3213-4295","position":7,"is_corresponding":false},{"id":1167157,"name":"Kristīne Oļeiņika","orcid":"0000-0002-2021-2938","position":0,"is_corresponding":true}],"reference_count":26,"raw_metadata":null,"created_at":"2026-07-19T02:53:41.314800Z","pmid":"41032891","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}