{"doi":"10.1093/jimmun/vkae061","title":"CD209d/e are required for macrophage-mediated phagocytosis and activation during methicillin-resistant <i>Staphylococcus aureus</i> pulmonary host defense","abstract":"Staphylococcus aureus is a commensal and opportunist pathogen of the upper respiratory tract. The recognition of pathogen-associated molecular patterns through pattern-recognition receptors is crucial for eliminating microorganisms such as S. aureus. DC-SIGN (CD209) is a pattern-recognition receptor that binds to a broad range of pathogens, promoting phagocytosis. Here we aimed to study the role of mouse homologues of DC-SIGN, CD209d/e, in a methicillin-resistant S. aureus (MRSA) pulmonary infection model. CD209d/e-/- and wild-type C57BL/6 mice were infected with MRSA and inflammatory parameters were evaluated. CD209d/e-/- mice had delayed bacterial burden and mortality together with increased frequency of neutrophils and decreased dendritic cells in the lung compared with control mice. iNOS+ macrophages, and regulatory T cell frequency were decreased in the lungs of CD209d/e-/- mice. CD209d/e-/- mice had increased levels of inflammatory cytokines in the lungs, but levels of IL-12p40 were decreased. MRSA reduced expression of interferon-γ and pattern-recognition receptors in CD209d/e-/- mice. MRSA uptake by phagocytes was decreased in the lungs of CD209d/e-/- versus control mice. CD209d/e-/- bone marrow derived macrophages showed impaired MRSA uptake and killing. These data suggest that CD209d/e are essential receptors to control inflammation by activating macrophages leading to MRSA uptake and killing.","journal":"The Journal of Immunology","year":2025,"id":543461,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1433092,"name":"Md. Sagir Ahmed","orcid":"0000-0002-8322-2041","position":1,"is_corresponding":false},{"id":296764,"name":"Michael A. Marinelli","orcid":null,"position":2,"is_corresponding":false},{"id":1135851,"name":"Leigh M. Miller","orcid":"0000-0001-6312-3946","position":3,"is_corresponding":false},{"id":501523,"name":"Alexis M. Duray","orcid":"0000-0002-1658-3701","position":4,"is_corresponding":false},{"id":1284952,"name":"Brooke P. Dresden","orcid":"0000-0001-9460-3985","position":5,"is_corresponding":false},{"id":1251931,"name":"Nicholas J Constantinesco","orcid":"0009-0007-1102-8251","position":6,"is_corresponding":false},{"id":1433093,"name":"Paul F. G. Sims","orcid":"0009-0008-8356-6398","position":7,"is_corresponding":false},{"id":1432675,"name":"Lacee J Richwalls","orcid":null,"position":8,"is_corresponding":false},{"id":818780,"name":"Saran Kupul","orcid":null,"position":9,"is_corresponding":false},{"id":246970,"name":"Jay K. Kolls","orcid":"0000-0001-5151-6304","position":10,"is_corresponding":false},{"id":295192,"name":"Radha Gopal","orcid":"0000-0003-0959-5744","position":11,"is_corresponding":false},{"id":295193,"name":"John F. Alcorn","orcid":"0000-0001-5997-7711","position":12,"is_corresponding":false},{"id":1284141,"name":"Flávia Rago","orcid":"0009-0003-1291-060X","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:53:04.088079Z","pmid":"40101753","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}