{"doi":"10.1093/jambio/lxaf145","title":"Long-term analysis of <i>Vibrio vulnificus</i> and <i>Vibrio parahaemolyticus</i> virulence factors and their environmental associations in the Chesapeake Bay, Maryland, US","abstract":"AIMS: The present study analyzed long-term trends in Vibrio spp. virulence factors associated with pathogenicity in Chesapeake Bay waters (in Maryland) across two 3-year sampling periods (2009-2012 and 2019-2022). METHODS AND RESULTS: Vibrio parahaemolyticus (n = 1476) and V. vulnificus (n = 806) isolates were recovered from 13 sampling stations and tested for species-specific and virulence markers. Across both sampling periods, detection of V. parahaemolyticus tdh+/trh+ isolates was similar and more frequently observed in the spring and fall, and the upper sampling stations. However, significant differences were observed in the detection of V. vulnificus pilA+ and rtxA+ isolates, with a higher percentage of pilA+ in 2019-2022 and rtxA+ in 2009-2012. VcgC+ isolates were most frequently detected during the fall (n = 24%) in 2019-2022. Interestingly, a greater prevalence of V. vulnificus isolates harboring virulence markers was observed in the mid and lower stations. CONCLUSIONS: Vibrio vulnificus isolates displayed greater changes in virulence marker frequency across sampling periods compared to V. parahaemolyticus and these markers were detectable throughout the year.","journal":"Journal of Applied Microbiology","year":2025,"id":544766,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.582,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":669666,"name":"Kyle D. Brumfield","orcid":"0000-0002-3234-3337","position":1,"is_corresponding":false},{"id":950950,"name":"Suhana Chattopadhyay","orcid":"0000-0003-4452-4810","position":2,"is_corresponding":false},{"id":950951,"name":"Leena Malayil","orcid":"0000-0002-8864-2678","position":3,"is_corresponding":false},{"id":669668,"name":"Anwar Huq","orcid":"0000-0003-0292-7021","position":4,"is_corresponding":false},{"id":37504,"name":"Rita R. Colwell","orcid":"0000-0001-5432-1502","position":5,"is_corresponding":false},{"id":950952,"name":"Amy R. Sapkota","orcid":"0000-0001-8666-6978","position":6,"is_corresponding":false},{"id":1047727,"name":"Michele E. Morgado","orcid":"0000-0001-7245-742X","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:53:17.418915Z","pmid":"40512518","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}