{"doi":"10.1093/jalm/jfaa023","title":"Development and Application of Mass Spectroscopy Assays for Nε-(1-Carboxymethyl)-L-Lysine and Pentosidine in Renal Failure and Diabetes","abstract":"BACKGROUND: Advanced glycation end products (AGEs) are formed via the nonenzymatic glycation of sugars with amino acids. Two AGEs, Nε-(1-carboxymethyl)-L-Lysine (CML) and pentosidine, have been observed to be elevated in subjects suffering from a multitude of chronic disease states, and accumulation of these compounds may be related to the pathophysiology of disease progression and aging. METHODS: We describe here the development and validation of a specific and reproducible LC-MS/MS method to quantify CML and pentosidine in human serum with lower limits of quantitation of 75 ng/mL and 5 ng/mL, respectively. The analyte calibration curve exhibited excellent linearity at a range of 0-10 900 ng/mL for CML and 0-800 ng/mL for pentosidine. High-low linearity of 5 serum pairs was assessed, with a mean recovery of 103% (range 94-116%) for CML, and 104% (range 97-116%) for pentosidine. RESULTS: Serum concentrations of CML and pentosidine were quantified in 30 control and 30 subjects with chronic renal insufficiency. A significant increase in both analytes was observed in renal failure compared to control subjects (2.1-fold and 8.4-fold, respectively; P < 0.001 for both). In a separate cohort of 49 control versus 95 subjects with type 2 diabetes mellitus (T2DM), serum CML but not serum pentosidine, was significantly elevated in the T2DM patients, and CML was also correlated with glycemic control, as assessed by hemoglobin A1c (r = 0.34, P < 0.001). CONCLUSIONS: These mass spectroscopy-based assays for serum CML and pentosidine should be useful in accurately evaluating circulating levels of these key AGEs in various disease states.","journal":"The Journal of Applied Laboratory Medicine","year":2020,"id":77866,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9649,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":233684,"name":"Sundeep Khosla","orcid":"0000-0002-2936-4372","position":1,"is_corresponding":false},{"id":241934,"name":"Joshua N. Farr","orcid":"0000-0002-3179-6414","position":2,"is_corresponding":false},{"id":265607,"name":"Olga P. Bondar","orcid":null,"position":3,"is_corresponding":false},{"id":97274,"name":"Elizabeth J. Atkinson","orcid":"0000-0002-1191-3775","position":4,"is_corresponding":false},{"id":300279,"name":"Sara J. Achenbach","orcid":null,"position":5,"is_corresponding":false},{"id":243921,"name":"Brittany Eckhardt","orcid":null,"position":6,"is_corresponding":false},{"id":243922,"name":"Brianne S Thicke","orcid":null,"position":7,"is_corresponding":false},{"id":243924,"name":"Amanda Tweed","orcid":null,"position":8,"is_corresponding":false},{"id":300280,"name":"Tammie L. Volkman","orcid":null,"position":9,"is_corresponding":false},{"id":241940,"name":"Matthew T. Drake","orcid":"0000-0002-3662-7345","position":10,"is_corresponding":false},{"id":265608,"name":"Jolaine M. Hines","orcid":null,"position":11,"is_corresponding":false},{"id":397360,"name":"Ravinder J. Singh","orcid":"0000-0003-4538-6544","position":12,"is_corresponding":false},{"id":87341,"name":"Katherine O’Grady","orcid":"0000-0001-6043-1070","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-18T21:49:16.197289Z","pmid":"32445362","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}