{"doi":"10.1093/jac/dkac051","title":"Predictors of 007 triphosphate concentrations in dried blood spots in persons with hepatitis C and active drug or alcohol use","abstract":"BACKGROUND: Sofosbuvir is converted to its active form, 007 triphosphate (007-TP), within cells. To date, the association between treatment adherence and 007-TP in dried blood spots (DBS) and factors that influence this relationship remain unknown. OBJECTIVES: To examine relationships between adherence and 007-TP concentrations in DBS and identify factors that influence 007-TP in DBS. METHODS: Persons with HCV or HIV/HCV coinfection and self-reported drug and/or alcohol use were randomized to one of two technology-based approaches for monitoring 12 week adherence to once-daily ledipasvir/sofosbuvir. Convenience blood samples were collected every 2 weeks during treatment. 007-TP in DBS was quantified using LC/MS and analysed using mixed-effects models. RESULTS: A total of 337 observations were available from 58 participants (78% male; 21% black; 22% Hispanic/Latino; 26% cirrhotic; 78% HIV-coinfected). The mean half-life of 007-TP in DBS was 142 h (95% CI 127-156) and concentrations increased by 7.3% (95% CI 2.2-12.6) for every 10% increase in between-visit adherence. Geometric mean (95% CI) 007-TP concentrations in DBS were 301 (247-368), 544 (462-639) and 647 (571-723) fmol/punch by adherence categories of ≤50%, >50 to ≤80%, and >80%. Adherence, time on therapy, increasing age and decreased estimated glomerular filtration rate were associated with higher 007-TP, whereas increased time since last dose, male sex, black race and higher BMI were associated with lower 007-TP. CONCLUSIONS: 007-TP has an extended half-life in DBS and concentrations increased with adherence. Further research is needed to examine additional factors that affect 007-TP and the clinical utility of this measure.","journal":"Journal of Antimicrobial Chemotherapy","year":2022,"id":291809,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9616,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":395985,"name":"José Castillo‐Mancilla","orcid":"0000-0003-1242-1745","position":1,"is_corresponding":false},{"id":395981,"name":"Mary Morrow","orcid":"0000-0002-2115-4864","position":2,"is_corresponding":false},{"id":395984,"name":"Samantha MaWhinney","orcid":"0000-0002-9818-0059","position":3,"is_corresponding":false},{"id":348514,"name":"Sarah E. Rowan","orcid":"0000-0003-2144-7410","position":4,"is_corresponding":false},{"id":414478,"name":"David Wyles","orcid":"0009-0000-3750-5924","position":5,"is_corresponding":false},{"id":414479,"name":"Joshua Blum","orcid":"0009-0001-5910-3034","position":6,"is_corresponding":false},{"id":415144,"name":"Ryan Huntley","orcid":null,"position":7,"is_corresponding":false},{"id":415145,"name":"Lana M. Salah","orcid":null,"position":8,"is_corresponding":false},{"id":415146,"name":"Arya Tehrani","orcid":null,"position":9,"is_corresponding":false},{"id":976008,"name":"Leah C. Jimmerson","orcid":null,"position":10,"is_corresponding":false},{"id":445413,"name":"Laura Roon","orcid":"0009-0000-4975-3343","position":11,"is_corresponding":false},{"id":280276,"name":"Lane R. Bushman","orcid":null,"position":12,"is_corresponding":false},{"id":279022,"name":"Peter L. Anderson","orcid":"0000-0002-1200-8494","position":13,"is_corresponding":false},{"id":355013,"name":"Jennifer J. Kiser","orcid":null,"position":14,"is_corresponding":false},{"id":414477,"name":"Kristina M. Brooks","orcid":"0000-0002-3832-8699","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-19T00:30:42.221508Z","pmid":"35194648","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}