{"doi":"10.1093/intimm/dxl135","title":"The expression of Wiskott-Aldrich syndrome protein (WASP) is dependent on WASP-interacting protein (WIP)","abstract":null,"journal":"International Immunology","year":2006,"id":613655,"datarank":0.5606504427425053,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"self_citation_contribution":0.5606504427425053,"citation_network_contribution":0.0,"self_endowment_contribution":0.5606504427425053,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1581028,"name":"M. Kirby","orcid":null,"position":1,"is_corresponding":false},{"id":1541311,"name":"S. A. Anderson","orcid":null,"position":2,"is_corresponding":false},{"id":1581030,"name":"P. L. Schwartzberg","orcid":null,"position":3,"is_corresponding":false},{"id":1581031,"name":"F. Candotti","orcid":null,"position":4,"is_corresponding":false},{"id":1581026,"name":"A. Konno","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The expression of Wiskott-Aldrich syndrome protein (WASP) is dependent on WASP-interacting protein (WIP)","abstract":"The Wiskott-Aldrich syndrome protein (WASP) is a key molecule for transduction of extracellular signals that induce a variety of critical biological events involving actin cytoskeleton rearrangement. Among the cellular partners of WASP, the Wiskott-Aldrich syndrome protein-interacting protein (WIP) has been speculated to play a critical role in the pathophysiology of Wiskott-Aldrich syndrome since WASP mutation hot spots map to the WIP-binding region. The notion that WIP promotes WASP function, however, conflicts with evidence that WIP inhibits WASP-mediated actin polymerization and IL-2 production and suggests a complex regulation of WASP function by WIP. Here we show that WASP gene transfer results in high WASP expression only when WIP is concomitantly expressed in K562 cells. Furthermore, WIP-knockdown experiments demonstrated that T cells with reduced WIP expression show a concordant reduction of WASP levels. Mapping studies using WIP mutants showed that the minimal WIP region able to rescue WASP expression in WIP-knockdown cells was the WASP-binding domain. However, expression of such a minimal domain of WIP failed to rescue WASP-dependent, nuclear factor of activated T-cells-mediated IL-2 transcriptional activity. These results demonstrate that expression of WIP is necessary for functional WASP expression in human cells and provide a new paradigm for understanding the function of these two molecules.","is_dataset_classified":null,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17205972","pmcid":null,"openalex_id":"https://openalex.org/W2154735536","authors":[],"funders":[{"funder_name":"Intramural NIH HHS","grant_id":"","title":null},{"funder_name":"Intramural NIH HHS","grant_id":"","title":null}],"total_grants":2,"fwci":1.2953,"citation_percentile":0.78042127,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":6},{"year":2014,"count":2},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2020,"count":2},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2025,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/intimm/article-pdf/19/2/185/1995039/dxl135.pdf","host_type":"journal"},{"url":"https://academic.oup.com/intimm/article-pdf/19/2/185/1995039/dxl135.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/intimm/article-pdf/19/2/185/1995039/dxl135.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/intimm/dxl135","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17205972","host_type":"repository"}],"fields_of_study":["Cellular Mechanics and Interactions","Cell Adhesion Molecules Research","Viral Infectious Diseases and Gene Expression in Insects","Blotting, Western","Carrier Proteins","Cell Line","Cytoskeletal Proteins","Flow Cytometry","Humans","Intracellular Signaling Peptides and Proteins","RNA Interference","Reverse Transcriptase Polymerase Chain Reaction","Transfection","Wiskott-Aldrich Syndrome Protein"],"mesh_terms":["Carrier Proteins","Cell Line","Cytoskeletal Proteins","Flow Cytometry","Humans","Transfection","Blotting, Western","Reverse Transcriptase Polymerase Chain Reaction","RNA Interference","Intracellular Signaling Peptides and Proteins","Wiskott-Aldrich Syndrome Protein"],"keywords":["Wiskott–Aldrich syndrome protein","Biology","Wiskott–Aldrich syndrome","Cell biology","Gene knockdown","Actin","Mutant","Actin cytoskeleton","Molecular biology","Gene","Cytoskeleton","Genetics","Cell"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T08:49:25.601909Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}