{"doi":"10.1093/infdis/jiy058","title":"Increased Virulence of an Encapsulated Streptococcus pneumoniae Upon Expression of Pneumococcal Surface Protein K","abstract":null,"journal":"The Journal of Infectious Diseases","year":2018,"id":645454,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1680706,"name":"Jessica L Bradshaw","orcid":null,"position":1,"is_corresponding":false},{"id":1680707,"name":"Lance E Keller","orcid":null,"position":2,"is_corresponding":false},{"id":1680708,"name":"Larry S McDaniel","orcid":null,"position":3,"is_corresponding":false},{"id":1680705,"name":"Haley R Pipkins","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Increased Virulence of an Encapsulated Streptococcus pneumoniae Upon Expression of Pneumococcal Surface Protein K","abstract":"Background: Current Streptococcus pneumoniae vaccines selectively target capsular polysaccharide of specific serotypes, leading to an increase in nonencapsulated S. pneumoniae (NESp). Cocolonization by encapsulated pneumococci and NESp increases the opportunity for intraspecies genetic exchange. Acquisition of NESp genes by encapsulated pneumococci could alter virulence and help vaccine-targeted serotypes persist in the host. Methods: Adhesion and invasion assays were performed using immortalized human pharyngeal or lung epithelial cells. In vivo models assessing murine nasopharyngeal colonization and pneumonia, as well as chinchilla otitis media (OM), were also used. Results: Pneumococcal surface protein K (PspK) expression increased encapsulated pneumococcal adhesion and invasion of lung cells and enhanced virulence during pneumonia and OM. Additionally, PspK increased nasopharyngeal colonization, persistence in the lungs, and persistence in the middle ear when expressed in a capsule deletion mutant. Competition experiments demonstrated encapsulated pneumococci expressing PspK also had a selective advantage in both the lungs and nasopharynx. Conclusions: PspK increases pneumococcal virulence during pneumonia and OM. PspK also partially compensates for loss of virulence in the absence of capsule. Additionally, PspK provides a selective advantage in a competitive environment. Therefore, acquisition of PspK increases encapsulated virulence in a condition-dependent manner. Together, these studies demonstrate risks associated with pneumococcal intraspecies genetic exchange.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29394357","pmcid":null,"openalex_id":"https://openalex.org/W2793868374","authors":[],"funders":[],"total_grants":0,"fwci":0.6009,"citation_percentile":0.67074352,"influential_citations":0,"citation_trend":[{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":3},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://academic.oup.com/journals/pages/about_us/legal/notices","oa_locations":[{"url":"https://academic.oup.com/jid/article-pdf/217/10/1637/24698527/jiy058.pdf","host_type":"journal"},{"url":"https://academic.oup.com/jid/article-pdf/217/10/1637/24698527/jiy058.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/jid/article-pdf/217/10/1637/24698527/jiy058.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/infdis/jiy058","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29394357","host_type":"repository"},{"url":"https://iris.unil.ch/handle/iris/135728","host_type":"repository"},{"url":"https://serval.unil.ch/notice/serval:BIB_D1D24682B260","host_type":"repository"}],"fields_of_study":["Pneumonia and Respiratory Infections","Bacterial Infections and Vaccines","vaccines and immunoinformatics approaches","A549 Cells","Animals","Bacterial Outer Membrane Proteins","Bacterial Proteins","Cell Line, Tumor","Humans","Lung","Mice","Mice, Inbred C57BL","Nasopharynx","Otitis Media","Pneumococcal Infections","Pneumococcal Vaccines","Serogroup","Streptococcus pneumoniae","Virulence","Virulence Factors"],"mesh_terms":["A549 Cells","Animals","Bacterial Outer Membrane Proteins","Bacterial Proteins","Humans","Lung","Mice, Inbred C57BL","Nasopharynx","Otitis Media","Pneumococcal Infections","Streptococcus pneumoniae","Virulence","Pneumococcal Vaccines","Virulence Factors","Cell Line, Tumor","Mice","Serogroup"],"keywords":["Virulence","Streptococcus pneumoniae","Pneumolysin","Microbiology","Biology","Pneumonia","Pneumococcal pneumonia","Pneumococcal infections","Serotype","Bacterial capsule","Immunology","Gene","Medicine","Antibiotics","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T06:03:57.803103Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}