{"doi":"10.1093/infdis/jit609","title":"Evaluation of Serologic and Antigenic Relationships Between Middle Eastern Respiratory Syndrome Coronavirus and Other Coronaviruses to Develop Vaccine Platforms for the Rapid Response to Emerging Coronaviruses","abstract":null,"journal":"The Journal of Infectious Diseases","year":2014,"id":639791,"datarank":5.957821779880165,"base_score":4.718498871295094,"endowment":4.718498871295094,"self_citation_contribution":0.7077748306942643,"citation_network_contribution":5.250046949185901,"self_endowment_contribution":0.7077748306942643,"citer_contribution":5.250046949185901,"corpus_percentile":null,"corpus_rank":null,"citation_count":111,"citer_count":93,"citers_with_citation_signal":86,"citers_with_endowment":86,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":526214,"name":"Robin Gopal","orcid":null,"position":1,"is_corresponding":false},{"id":105503,"name":"Boyd L. Yount","orcid":"0000-0002-6099-8429","position":2,"is_corresponding":false},{"id":1662541,"name":"Eric F. Donaldson","orcid":null,"position":3,"is_corresponding":false},{"id":105316,"name":"Vineet D. Menachery","orcid":"0000-0001-8803-7606","position":4,"is_corresponding":false},{"id":103705,"name":"Rachel L. Graham","orcid":"0000-0002-3143-6515","position":5,"is_corresponding":false},{"id":1662542,"name":"Trevor D. Scobey","orcid":null,"position":6,"is_corresponding":false},{"id":105508,"name":"Lisa E. Gralinski","orcid":"0000-0003-1374-8002","position":7,"is_corresponding":false},{"id":105126,"name":"Mark R. Denison","orcid":"0000-0003-2655-0900","position":8,"is_corresponding":false},{"id":70962,"name":"Maria Zambon","orcid":"0000-0002-8897-7881","position":9,"is_corresponding":false},{"id":5952,"name":"Ralph S. Baric","orcid":"0000-0001-6827-8701","position":10,"is_corresponding":false},{"id":300195,"name":"Sudhakar Agnihothram","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Evaluation of Serologic and Antigenic Relationships Between Middle Eastern Respiratory Syndrome Coronavirus and Other Coronaviruses to Develop Vaccine Platforms for the Rapid Response to Emerging Coronaviruses","abstract":"BACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012, causing severe acute respiratory disease and pneumonia, with 44% mortality among 136 cases to date. Design of vaccines to limit the virus spread or diagnostic tests to track newly emerging strains requires knowledge of antigenic and serologic relationships between MERS-CoV and other CoVs. METHODS: Using synthetic genomics and Venezuelan equine encephalitis virus replicons (VRPs) expressing spike and nucleocapsid proteins from MERS-CoV and other human and bat CoVs, we characterize the antigenic responses (using Western blot and enzyme-linked immunosorbent assay) and serologic responses (using neutralization assays) against 2 MERS-CoV isolates in comparison with those of other human and bat CoVs. RESULTS: Serologic and neutralization responses against the spike glycoprotein were primarily strain specific, with a very low level of cross-reactivity within or across subgroups. CoV N proteins within but not across subgroups share cross-reactive epitopes with MERS-CoV isolates. Our findings were validated using a convalescent-phase serum specimen from a patient infected with MERS-CoV (NA 01) and human antiserum against SARS-CoV, human CoV NL63, and human CoV OC43. CONCLUSIONS: Vaccine design for emerging CoVs should involve chimeric spike protein containing neutralizing epitopes from multiple virus strains across subgroups to reduce immune pathology, and a diagnostic platform should include a panel of nucleocapsid and spike proteins from phylogenetically distinct CoVs.","is_dataset_classified":null,"base_score":4.718498871295094,"endowment":4.718498871295094,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24253287","pmcid":"PMC3952667","openalex_id":"https://openalex.org/W2100790445","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"AI057157","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI085524","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI085524","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"U19 AI107810","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"U54 AI057157","title":null},{"funder_name":"National Institutes of Health","grant_id":"5U19AI107810-02","title":"Characterization of novel genes encoded ty RNA and DNA viruses"}],"total_grants":6,"fwci":4.3964,"citation_percentile":0.9529896,"influential_citations":0,"citation_trend":[{"year":2014,"count":12},{"year":2015,"count":5},{"year":2016,"count":9},{"year":2017,"count":5},{"year":2018,"count":3},{"year":2019,"count":8},{"year":2020,"count":34},{"year":2021,"count":13},{"year":2022,"count":8},{"year":2023,"count":7},{"year":2024,"count":4},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/jid/article-pdf/209/7/995/13804699/jit609.pdf","host_type":"journal"},{"url":"https://academic.oup.com/jid/article-pdf/209/7/995/13804699/jit609.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/jid/article-pdf/209/7/995/13804699/jit609.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/infdis/jit609","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24253287","host_type":"repository"},{"url":"https://cdr.lib.unc.edu/concern/articles/m326m946f","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.893.9060","host_type":""},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.898.5603","host_type":""},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3952667","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3952667","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3952667?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1093/infdis/jit609","host_type":""},{"url":"https://dx.doi.org/10.17615/zdkg-xj11","host_type":""},{"url":"https://dx.doi.org/10.1093/infdis/jit609","host_type":""}],"fields_of_study":["SARS-CoV-2 and COVID-19 Research","Animal Virus Infections Studies","vaccines and immunoinformatics approaches","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Coronavirus Nucleocapsid Proteins","Animals","Antibodies, Viral","Antigens, Viral","Chiroptera","Coronaviridae","Cross Reactions","Enzyme-Linked Immunosorbent Assay","Humans","Mice, Inbred BALB C","Middle Aged","Neutralization Tests","Blotting, Western","Nucleocapsid Proteins","Mice","Spike Glycoprotein, Coronavirus"],"keywords":["Virology","Middle East respiratory syndrome coronavirus","Coronavirus","Epitope","Biology","Serology","Middle East respiratory syndrome","Antigen","Betacoronavirus","Coronaviridae","Virus","Plaque reduction neutralization test","Immunodominance","Neutralization","Antibody","Immunology","Disease","Medicine","Infectious disease (medical specialty)","Coronavirus disease 2019 (COVID-19)","Diagnostics","Synthetic Genomics","Mers-cov Vaccine Design","Mice, Inbred BALB C","Blotting, Western","Enzyme-Linked Immunosorbent Assay","Cross Reactions","Middle Aged","Nucleocapsid Proteins","Antibodies, Viral","Major Articles and Brief Reports","Mice","Infectious Diseases","Neutralization Tests","Chiroptera","Spike Glycoprotein, Coronavirus","Animals","Coronavirus Nucleocapsid Proteins","Humans","Immunology and Allergy","Antigens, Viral"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"},{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T01:47:21.635919Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}