{"doi":"10.1093/infdis/jiaf474","title":"Febrile Temperature Augments Ring-stage <i>Plasmodium falciparum</i> Adhesion to Brain Endothelial Cells","abstract":"Sequestration of Plasmodium falciparum-infected erythrocytes (IE) in the microvasculature is a major virulence determinant. While the sequestration of mature stage parasites (trophozoite and schizonts) to vascular endothelium is well established, the conditions that promote ring-stage IE sequestration are less understood. Here, we observed in ring-stage parasites that febrile exposure increased transcript levels of several exported parasite genes involved in the trafficking of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) ligand responsible for adherence to the endothelium of blood vessels. Furthermore, it accelerated PfEMP1 surface display in ring-stage IEs, leading to a 2-fold increase in their binding in a perfusable 3D human brain microvessel model. Additionally, we observed that parasite exposure enhances the binding of uninfected erythrocytes in 3D brain microvessels. These findings suggest a complex interplay between fever and parasite biomass in the pathogenesis of cerebral malaria.","journal":"The Journal of Infectious Diseases","year":2025,"id":574574,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9586,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":820823,"name":"Ruoqian Hu","orcid":"0009-0002-6103-4063","position":1,"is_corresponding":false},{"id":75973,"name":"Karl B. Seydel","orcid":"0000-0001-9859-9549","position":2,"is_corresponding":false},{"id":260116,"name":"Lauren M. Cohee","orcid":"0000-0002-7575-630X","position":3,"is_corresponding":false},{"id":281119,"name":"Ying Zheng","orcid":"0000-0003-2723-9022","position":4,"is_corresponding":false},{"id":281118,"name":"Joseph D. Smith","orcid":"0000-0002-7915-6360","position":5,"is_corresponding":false},{"id":1063750,"name":"Fatou Joof","orcid":"0009-0002-9731-9239","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-19T02:57:44.572630Z","pmid":"40929367","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}