{"doi":"10.1093/infdis/jiae514","title":"Evaluation of Immunoglobulin A Enzyme Immunoassays to Detect Primary Respiratory Syncytial Virus Infection in Infants and Young Children","abstract":"BACKGROUND: Respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory infections in children <2 years of age. Prior infection in a child is usually determined by RSV antibodies; however, in young children, persisting maternal immunoglobulin G antibodies can incorrectly indicate past RSV infection. We developed and evaluated 4 immunoglobulin A (IgA) antibody enzyme immunoassays (EIAs) with the RSV F, subgroup G (Ga or Gb proteins) or RSV lysate antigens to distinguish infection induced from persisting maternal RSV antibodies. METHODS: We tested the EIAs against 62 cord blood specimens (group A), 39 plasma specimens from infants not exposed to an RSV season (group B), 102 plasma specimens from infants with a documented RSV infection (group C), and 124 plasma specimens from infants exposed to their first RSV season but without a documented RSV infection (group D). RESULTS: Among the 2 negative control groups, no group A specimens and 1 of the group B specimens were positive in all 4 IgA EIAs, giving a specificity of 100% and 97%, respectively. The sensitivity of the F, Ga, Gb, and Lysate IgA EIAs were 88%, 31%, 26%, and 61%, respectively, for group C specimens. Forty-four percent of the 124 specimens in group D were positive in the RSV-F IgA EIA. CONCLUSIONS: The RSV-F protein IgA EIA exhibited a high level of sensitivity and specificity for detecting previous RSV infections in the presence of maternal antibodies and can help in RSV clinical trials and epidemiologic studies in young children.","journal":"The Journal of Infectious Diseases","year":2024,"id":482331,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9621,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":421437,"name":"Binh Ha","orcid":null,"position":1,"is_corresponding":false},{"id":1322559,"name":"Heying Sun","orcid":"0009-0008-6466-6430","position":2,"is_corresponding":false},{"id":1225361,"name":"Katie Liu","orcid":"0000-0002-5687-8969","position":3,"is_corresponding":false},{"id":421438,"name":"Samadhan Jadhao","orcid":null,"position":4,"is_corresponding":false},{"id":94483,"name":"Laila Hussaini","orcid":null,"position":5,"is_corresponding":false},{"id":416567,"name":"Courtney McCracken","orcid":"0000-0001-7941-1256","position":6,"is_corresponding":false},{"id":616975,"name":"Theda Gibson","orcid":null,"position":7,"is_corresponding":false},{"id":409698,"name":"İnci Yıldırım","orcid":"0000-0002-8631-0020","position":8,"is_corresponding":false},{"id":664172,"name":"Jumi Yi","orcid":null,"position":9,"is_corresponding":false},{"id":616980,"name":"Kathy Stephens","orcid":null,"position":10,"is_corresponding":false},{"id":1322904,"name":"Chelsea Korski","orcid":null,"position":11,"is_corresponding":false},{"id":338069,"name":"Carol Kao","orcid":null,"position":12,"is_corresponding":false},{"id":94488,"name":"Christina A. Rostad","orcid":"0000-0001-8439-0592","position":13,"is_corresponding":false},{"id":94484,"name":"Evan J. Anderson","orcid":"0000-0002-1576-4420","position":14,"is_corresponding":false},{"id":232941,"name":"Larry J. Anderson","orcid":"0000-0002-0652-6834","position":15,"is_corresponding":false},{"id":1322558,"name":"Ranjini Sankaranarayanan","orcid":"0000-0003-3562-4860","position":0,"is_corresponding":true}],"reference_count":29,"raw_metadata":null,"created_at":"2026-07-19T02:07:14.349221Z","pmid":"39575588","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}