{"doi":"10.1093/infdis/jiaa531","title":"A High-Throughput Assay for Circulating Antibodies Directed Against the S Protein of Severe Acute Respiratory Syndrome Coronavirus 2","abstract":"More than 24 million infections with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were confirmed globally by September 2020. While polymerase chain reaction-based assays are used for diagnosis, there is a need for high-throughput, rapid serologic methods. A Luminex binding assay was developed and used to assess simultaneously the presence of coronavirus disease 2019 (COVID-19)-specific antibodies in human serum and plasma. Clear differentiation was achieved between specimens from infected and uninfected subjects, and a wide range of serum/plasma antibody levels was delineated in infected subjects. All 25 specimens from 18 patients with COVID-19 were positive in the assays with both the trimeric spike and the receptor-binding domain proteins. None of the 13 specimens from uninfected subjects displayed antibodies to either antigen. There was a highly statistically significant difference between the antibody levels of COVID-19-infected and -uninfected specimens (P < .0001). This high-throughput antibody assay is accurate, requires only 2.5 hours, and uses 5 ng of antigen per test.","journal":"The Journal of Infectious Diseases","year":2020,"id":96669,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":291517,"name":"Jéromine Klingler","orcid":"0000-0002-2313-9932","position":1,"is_corresponding":false},{"id":291527,"name":"Catarina E. Hioe","orcid":"0000-0002-9245-9788","position":2,"is_corresponding":false},{"id":104911,"name":"Fatima Amanat","orcid":"0000-0002-8029-8227","position":3,"is_corresponding":false},{"id":291359,"name":"Ian Baine","orcid":"0000-0002-0419-0054","position":4,"is_corresponding":false},{"id":291524,"name":"Suzanne Arinsburg","orcid":"0000-0002-0739-9152","position":5,"is_corresponding":false},{"id":104926,"name":"Erna Milunka Kojic","orcid":null,"position":6,"is_corresponding":false},{"id":104927,"name":"Jonathan Stoever","orcid":"0000-0003-1995-101X","position":7,"is_corresponding":false},{"id":104928,"name":"Sean Liu","orcid":"0000-0002-3842-6838","position":8,"is_corresponding":false},{"id":104919,"name":"Denise Jurczyszak","orcid":"0000-0003-4634-5672","position":9,"is_corresponding":false},{"id":104921,"name":"Maria C. Bermúdez‐González","orcid":"0000-0002-7908-6286","position":10,"is_corresponding":false},{"id":104936,"name":"Viviana Simon","orcid":"0000-0002-6416-5096","position":11,"is_corresponding":false},{"id":103779,"name":"Florian Krammer","orcid":"0000-0003-4121-776X","position":12,"is_corresponding":false},{"id":291526,"name":"Susan Zolla‐Pazner","orcid":"0000-0002-0750-2666","position":13,"is_corresponding":false},{"id":291518,"name":"Svenja Weiß","orcid":"0000-0003-4136-0566","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":null,"created_at":"2026-07-18T22:35:06.014648Z","pmid":"32860510","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}