{"doi":"10.1093/glycob/9.7.723","title":"Characterization of fibroblast growth factor 1 binding heparan sulfate domain","abstract":null,"journal":"Glycobiology","year":1999,"id":634786,"datarank":0.6663976884735476,"base_score":4.442651256490317,"endowment":4.442651256490317,"self_citation_contribution":0.6663976884735476,"citation_network_contribution":0.0,"self_endowment_contribution":0.6663976884735476,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":84,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1646556,"name":"J Kreuger","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Characterization of fibroblast growth factor 1 binding heparan sulfate domain","abstract":"Fibroblast growth factors FGF-1 and FGF-2 mediate their biological effects via heparan sulfate-dependent interactions with cell surface FGF receptors. While the specific heparan sulfate domain binding to FGF-2 has been elucidated in some detail, limited information has been available concerning heparan sulfate structures involved in the recognition of FGF-1. In the current study we present evidence that the minimal FGF-1 binding heparan sulfate sequence comprises 5-7 monosaccharide units and contains a critical trisulfated IdoA(2-OSO3)-GlcNSO3(6-OSO3) disaccharide unit. N-Sulfated heparan sulfate decasaccharides depleted of FGF-1 binding domains showed dose-dependent and saturable binding to FGF-2. These data indicate that the FGF-1 binding domain is distinct from the minimal FGF-2 binding site, previously shown to contain an IdoA(2-OSO3) residue but no 6-O-sulfate groups. We further show that the FGF-1 binding heparan sulfate domain is expressed in human aorta heparan sulfate in an age-related manner in contrast to the constitutively expressed FGF-2 binding domain. Reduction of heparan sulfate O-sulfation by chlorate treatment of cells selectively impedes binding to FGF-1. The present data implicate the 6-O-sulfation of IdoA(2-OSO3)-GlcNSO3 units in cellular heparan sulfate in the control of the biological activity of FGF-1.","is_dataset_classified":null,"base_score":4.442651256490317,"endowment":4.442651256490317,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10362842","pmcid":null,"openalex_id":"https://openalex.org/W2116184270","authors":[],"funders":[],"total_grants":0,"fwci":2.9362,"citation_percentile":0.91291206,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":1},{"year":2014,"count":5},{"year":2015,"count":1},{"year":2016,"count":3},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/glycob/article-pdf/9/7/723/1965219/9-7-723.pdf","host_type":"journal"},{"url":"https://academic.oup.com/glycob/article-pdf/9/7/723/1965219/9-7-723.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/glycob/article-pdf/9/7/723/1965219/9-7-723.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/glycob/9.7.723","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10362842","host_type":"repository"}],"fields_of_study":["Proteoglycans and glycosaminoglycans research","Fibroblast Growth Factor Research","Connective tissue disorders research","Animals","Binding Sites","Carbohydrate Sequence","Cattle","Fibroblast Growth Factor 1","Fibroblast Growth Factor 2","Heparan Sulfate","Humans","In Vitro Techniques","Molecular Sequence Data","Recombinant Proteins","Swine"],"mesh_terms":["Animals","Binding Sites","Carbohydrate Sequence","Cattle","Heparan Sulfate","Heparitin Sulfate","Humans","Molecular Sequence Data","Recombinant Proteins","Swine","Fibroblast Growth Factor 1","Fibroblast Growth Factor 2","In Vitro Techniques"],"keywords":["Heparan sulfate","Fibroblast growth factor","Sulfation","Chemistry","Perlecan","Biochemistry","Iduronic acid","Binding site","Glycosaminoglycan","Receptor"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T14:10:26.403330Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}