{"doi":"10.1093/glycob/2.4.369","title":"Topology of ER processing α-mannosidase of <i>Saccharomyces cerevisae</i>","abstract":null,"journal":"Glycobiology","year":1992,"id":618660,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1584315,"name":"Annette Herscovics","orcid":null,"position":1,"is_corresponding":false},{"id":1596159,"name":"Benoît Grondin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Topology of ER processing α-mannosidase of <i>Saccharomyces cerevisae</i>","abstract":"The yeast specific alpha-mannosidase which converts Man9GlcNAc to a single isomer of Man8GlcNAc is involved in N-linked oligosaccharide processing in the endoplasmic reticulum (ER). Sequence analysis of the structural gene for this enzyme suggested that it is a type II transmembrane protein (Camirand et al., 1991). To firmly establish its membrane topology, the gene was transcribed in vitro and translation was performed in a reticulocyte lysate with and without dog pancreas microsomal membranes. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of [35S]methionine-labelled products showed that the largest band formed corresponded in size to the 63 kDa peptide expected from the alpha-mannosidase gene product. It was transformed into a 4 kDa larger endoglycosidase H-sensitive band in the presence of microsomal membranes. This glycosylated translation product was completely protected from proteinase K digestion in the absence of detergent. These results demonstrate that the yeast ER alpha-mannosidase is a type II membrane protein, like Golgi enzymes involved in N-linked glycosylation.","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1421758","pmcid":null,"openalex_id":"https://openalex.org/W1991405766","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM-31265","title":null}],"total_grants":1,"fwci":0.4119,"citation_percentile":0.59475287,"influential_citations":0,"citation_trend":[{"year":2014,"count":2},{"year":2020,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://academic.oup.com/glycob/article-pdf/2/4/369/1740263/2-4-369.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/glycob/2.4.369","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1421758","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Glycosylation and Glycoproteins Research","Lysosomal Storage Disorders Research"],"mesh_terms":["Animals","Dogs","Electrophoresis, Polyacrylamide Gel","Endoplasmic Reticulum","Glycosylation","Hexosaminidases","Intracellular Membranes","Mannosidases","Microsomes","Oligosaccharides","Pancreas","Saccharomyces cerevisiae","Serine Endopeptidases","Transcription, Genetic","Protein Biosynthesis","Endopeptidase K","alpha-Mannosidase"],"keywords":["Mannosidase","Endoplasmic reticulum","Endoglycosidase H","Membrane topology","Reticulocyte","Biochemistry","Golgi apparatus","Glycosylation","Saccharomyces cerevisiae","Membrane protein","Proteinase K","Biology","Chemistry","Molecular biology","Yeast","Enzyme","Gene","Membrane","Messenger RNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T04:57:14.885275Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}