{"doi":"10.1093/gigascience/giaa156","title":"Identifying the effect of vancomycin on health care–associated methicillin-resistant <i>Staphylococcus aureus</i> strains using bacteriological and physiological media","abstract":"BACKGROUND: The evolving antibiotic-resistant behavior of health care-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more. FINDINGS: The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments. CONCLUSIONS: The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.","journal":"GigaScience","year":2021,"id":181803,"datarank":0.5476771790846133,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.13178887074864615,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.13178887074864615,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":9,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9647,"is_data_producer":true,"deposit_databanks":{"figshare":["10.6084/m9.figshare.12246251","10.6084/m9.figshare.12246257","10.6084/m9.figshare.12246248"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":559193,"name":"Saugat Poudel","orcid":"0000-0002-3732-2463","position":1,"is_corresponding":false},{"id":442184,"name":"Hannah Tsunemoto","orcid":"0000-0002-1546-097X","position":2,"is_corresponding":false},{"id":225251,"name":"Michael J. Meehan","orcid":"0000-0002-6836-0294","position":3,"is_corresponding":false},{"id":442185,"name":"Richard Szubin","orcid":"0000-0002-1212-8728","position":4,"is_corresponding":false},{"id":559196,"name":"Connor A. Olson","orcid":"0000-0003-3681-0446","position":5,"is_corresponding":false},{"id":314812,"name":"Yara Seif","orcid":"0000-0001-8813-5679","position":6,"is_corresponding":false},{"id":264948,"name":"Anne Lamsa","orcid":null,"position":7,"is_corresponding":false},{"id":442183,"name":"Nicholas Dillon","orcid":"0000-0003-2351-0700","position":8,"is_corresponding":false},{"id":225237,"name":"Alison Vrbanac","orcid":"0000-0001-5675-5515","position":9,"is_corresponding":false},{"id":459024,"name":"Joseph Sugie","orcid":"0000-0003-2911-1807","position":10,"is_corresponding":false},{"id":442186,"name":"Samira Dahesh","orcid":"0009-0006-4451-2034","position":11,"is_corresponding":false},{"id":5439,"name":"Jonathan M. Monk","orcid":"0000-0002-3895-8949","position":12,"is_corresponding":false},{"id":3553,"name":"Pieter C. Dorrestein","orcid":"0000-0002-3003-1030","position":13,"is_corresponding":false},{"id":3554,"name":"Rob Knight","orcid":"0000-0002-0975-9019","position":14,"is_corresponding":false},{"id":244710,"name":"Joe Pogliano","orcid":"0000-0001-8989-2655","position":15,"is_corresponding":false},{"id":225262,"name":"Victor Nizet","orcid":"0000-0003-3847-0422","position":16,"is_corresponding":false},{"id":225829,"name":"Adam M. Feist","orcid":"0000-0002-8630-4800","position":17,"is_corresponding":false},{"id":5441,"name":"Bernhard Ø. Palsson","orcid":"0000-0003-2357-6785","position":18,"is_corresponding":false},{"id":567001,"name":"Akanksha Rajput","orcid":"0000-0001-8176-6697","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-18T23:48:05.146883Z","pmid":"33420779","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}