{"doi":"10.1093/genetics/iyag022","title":"A stress-dependent postembryonic role for the core CPA factor CFIM-1 in germline integrity","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Post-transcriptional processing of pre-mRNAs by alternative polyadenylation (APA) generates a diversity of transcript isoforms at the 3′ untranslated region (3′UTR) that can affect their function and stability. The differential enrichment of transcript isoforms has been implicated in diseases ranging from cancer to neurodevelopmental disorders. However, the postembryonic developmental roles of the core ensemble of cleavage and polyadenylation (CPA) factors that mediate these post-transcriptional changes remain poorly characterized. Here, we report a stress-dependent role for the core CPA factor CFIM-1 in Caenorhabditis elegans germline integrity. Total loss-of-function of cfim-1 elicits a temperature-sensitive sterility phenotype in hermaphrodites but CFIM-1 protein levels do not change with temperature. Sterility is accompanied by sperm, oocyte, and germline morphology defects. Surveying the transcriptome of cfim-1(lf) worms revealed changes in transcript isoform abundance for dozens of genes with functions related to the development and maintenance of these structures. Collectively, our findings define a postembryonic role for a core CPA factor in tissue-specific development.</jats:p>","journal":"GENETICS","year":2026,"id":612225,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1322719,"name":"Rhea Ahluwalia","orcid":null,"position":1,"is_corresponding":false},{"id":1163319,"name":"Bin Yu","orcid":"0000-0002-2453-7852","position":2,"is_corresponding":false},{"id":1576215,"name":"Claudia Makhanko-Tang","orcid":null,"position":3,"is_corresponding":false},{"id":1576216,"name":"Chien-Hui Chang","orcid":null,"position":4,"is_corresponding":false},{"id":1576217,"name":"Haruka Nishimura","orcid":null,"position":5,"is_corresponding":false},{"id":1576218,"name":"Alexander Leong","orcid":null,"position":6,"is_corresponding":false},{"id":1576219,"name":"Ka Nam Naomi Kwan","orcid":null,"position":7,"is_corresponding":false},{"id":661430,"name":"W. Brent Derry","orcid":"0000-0002-1977-8475","position":8,"is_corresponding":false},{"id":1100396,"name":"Anson Sathaseevan","orcid":"0000-0002-6897-873X","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A stress-dependent postembryonic role for the core CPA factor CFIM-1 in germline integrity","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Post-transcriptional processing of pre-mRNAs by alternative polyadenylation (APA) generates a diversity of transcript isoforms at the 3′ untranslated region (3′UTR) that can affect their function and stability. The differential enrichment of transcript isoforms has been implicated in diseases ranging from cancer to neurodevelopmental disorders. However, the postembryonic developmental roles of the core ensemble of cleavage and polyadenylation (CPA) factors that mediate these post-transcriptional changes remain poorly characterized. Here, we report a stress-dependent role for the core CPA factor CFIM-1 in Caenorhabditis elegans germline integrity. Total loss-of-function of cfim-1 elicits a temperature-sensitive sterility phenotype in hermaphrodites but CFIM-1 protein levels do not change with temperature. Sterility is accompanied by sperm, oocyte, and germline morphology defects. Surveying the transcriptome of cfim-1(lf) worms revealed changes in transcript isoform abundance for dozens of genes with functions related to the development and maintenance of these structures. Collectively, our findings define a postembryonic role for a core CPA factor in tissue-specific development.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41606827","pmcid":null,"openalex_id":"https://openalex.org/W7125946910","authors":[],"funders":[{"funder_name":"Canadian Institutes of Health Research","grant_id":"PJT 155928","title":null},{"funder_name":"Canadian Institutes of Health Research","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Canada Research Chair in Animal Models of Human Disease","grant_id":"","title":null}],"total_grants":3,"fwci":0.0,"citation_percentile":0.07886952,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"OUP Standard Publication Reuse","oa_locations":[{"url":"https://academic.oup.com/genetics/advance-article-pdf/doi/10.1093/genetics/iyag022/66618414/iyag022.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/genetics/article-pdf/232/4/iyag022/66618414/iyag022.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/genetics/iyag022","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41606827","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","Connective tissue disorders research","Cancer-related gene regulation","Medicine","Biology","0301 basic medicine","03 medical and health sciences"],"mesh_terms":["Animals","Female","Germ Cells","Male","Stress, Physiological","Caenorhabditis elegans","Gene Expression Regulation, Developmental","Polyadenylation","Caenorhabditis elegans Proteins","mRNA Cleavage and Polyadenylation Factors"],"keywords":["Polyadenylation","Germline","Caenorhabditis elegans","Gene isoform","Phenotype","Sterility","Untranslated region","Gene","Apoptosis","Stress response","Cleavage And Polyadenylation","Wormbase","Nudt21","Cfim25","Cfim-1","Caenorhabditis Elegans; Alternative Polyadenylation"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T02:17:16.537703Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}