{"doi":"10.1093/genetics/136.4.1325","title":"Identification of DNA segments capable of rescuing a non-mendelian mutant in paramecium.","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>The non-Mendelian mutant d48 of Paramecium tetraurelia contains micronuclear wild type A genes, but at autogamy and conjugation proper processing fails and new macronuclei lack A genes. When cloned A genes are injected into the macronucleus of d48, proper processing is restored at the next autogamy; d48 is rescued, becoming permanently wild type. In the present study we have injected portions of the A gene into d48. We find that the ability to rescue extends over a large portion of the gene, with highest activity near a series of 221-bp repeat units in the middle of the gene. Regions outside the A gene are inactive.</jats:p>","journal":"Genetics","year":1994,"id":596947,"datarank":0.5289540786924243,"base_score":3.5263605246161616,"endowment":3.5263605246161616,"self_citation_contribution":0.5289540786924243,"citation_network_contribution":0.0,"self_endowment_contribution":0.5289540786924243,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1529022,"name":"J R Preer","orcid":null,"position":1,"is_corresponding":false},{"id":1529023,"name":"B Polisky","orcid":null,"position":2,"is_corresponding":false},{"id":1529021,"name":"C S Kim","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Identification of DNA segments capable of rescuing a non-mendelian mutant in paramecium.","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>The non-Mendelian mutant d48 of Paramecium tetraurelia contains micronuclear wild type A genes, but at autogamy and conjugation proper processing fails and new macronuclei lack A genes. When cloned A genes are injected into the macronucleus of d48, proper processing is restored at the next autogamy; d48 is rescued, becoming permanently wild type. In the present study we have injected portions of the A gene into d48. We find that the ability to rescue extends over a large portion of the gene, with highest activity near a series of 221-bp repeat units in the middle of the gene. Regions outside the A gene are inactive.</jats:p>","is_dataset_classified":null,"base_score":3.5263605246161616,"endowment":3.5263605246161616,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8013909","pmcid":"PMC1205913","openalex_id":"https://openalex.org/W2140441397","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM 31745","title":null}],"total_grants":1,"fwci":1.6381,"citation_percentile":0.84193803,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2023,"count":1}],"oa_status":"bronze","license":"https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model","oa_locations":[{"url":"https://academic.oup.com/genetics/article-pdf/136/4/1325/34596800/genetics1325.pdf","host_type":"journal"},{"url":"https://academic.oup.com/genetics/article-pdf/136/4/1325/34596800/genetics1325.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/genetics/article-pdf/136/4/1325/34596800/genetics1325.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/genetics/136.4.1325","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8013909","host_type":"repository"}],"fields_of_study":["Protist diversity and phylogeny","Microbial Community Ecology and Physiology","Genomics and Phylogenetic Studies"],"mesh_terms":["Animals","Antigens, Protozoan","Antigens, Surface","Mutation","Paramecium","Repetitive Sequences, Nucleic Acid","Protozoan Proteins","DNA, Protozoan","Genes, Protozoan"],"keywords":["Macronucleus","Biology","Mutant","Gene","Genetics","Paramecium","Mendelian inheritance","DNA","Mutation","Cell biology"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T11:48:30.260719Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}