{"doi":"10.1093/g3journal/jkaf129","title":"A reference genome for <i>Trichogramma kaykai</i>: a tiny desert-dwelling parasitoid wasp with competing sex-ratio distorters","abstract":"The tiny parasitoid wasp Trichogramma kaykai inhabits the Mojave Desert of the southwest United States. Populations of this tiny insect variably host up to 2 different sex-distorting genetic elements: (1) the endosymbiotic bacterium Wolbachia which induces the parthenogenetic reproduction of females, and (2) a B-chromosome, \"Paternal Sex Ratio\" (PSR), which converts would-be female offspring to PSR-transmitting males. We report here the genome of a Wolbachia-infected T. kaykai isofemale colony KSX58. Using Oxford Nanopore sequencing, we produced a final genome assembly of 205 Mbp with 34× coverage, consisting of 154 contigs with an N50 of 2.2 Mbp. The assembly is quite complete, with 92.67% complete Hymenoptera BUSCOs recovered: a very high score for Trichogrammatids that have been previously characterized for having high levels of core gene losses. We also report a complete mitochondrial genome for T. kaykai, and an assembly of the associated Wolbachia, strain wTkk. Finally, we identified copies of the parthenogenesis-inducing (PI) genes pifA and pifB in a remnant prophage region of the wTkk genome and compared their evolution to pifs from a suite of other PI Wolbachia. The T. kaykai assembly is one of the highest quality genome assemblies for the genus to date and will serve as a great resource for understanding the evolution of sex and selfish genetic elements.","journal":"G3 Genes Genomes Genetics","year":2025,"id":525931,"datarank":0.21345046354626357,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0055063093782799395,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0055063093782799395,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":2,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.6712,"is_data_producer":true,"deposit_databanks":{"BioProject":["PRJNA1150630"],"SRA":["SRR30339640"],"GENBANK":["JBJJXK000000000","JBJJXI000000000","PQ667799"],"figshare":["10.25387/g3.29226122"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":456317,"name":"Amelia R. I. Lindsey","orcid":"0000-0003-3354-7419","position":1,"is_corresponding":false},{"id":1358048,"name":"Jack A Culotta","orcid":null,"position":0,"is_corresponding":true}],"reference_count":95,"raw_metadata":null,"created_at":"2026-07-19T02:50:25.860105Z","pmid":"40476364","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}