{"doi":"10.1093/function/zqad062","title":"Coordinated Regulation of Myonuclear DNA Methylation, mRNA, and miRNA Levels Associates With the Metabolic Response to Rapid Synergist Ablation-Induced Skeletal Muscle Hypertrophy in Female Mice","abstract":"The central dogma of molecular biology dictates the general flow of molecular information from DNA that leads to a functional cellular outcome. In skeletal muscle fibers, the extent to which global myonuclear transcriptional alterations, accounting for epigenetic and post-transcriptional influences, contribute to an adaptive stress response is not clearly defined. In this investigation, we leveraged an integrated analysis of the myonucleus-specific DNA methylome and transcriptome, as well as myonuclear small RNA profiling to molecularly define the early phase of skeletal muscle fiber hypertrophy. The analysis of myonucleus-specific mature microRNA and other small RNA species provides new directions for exploring muscle adaptation and complemented the methylation and transcriptional information. Our integrated multi-omics interrogation revealed a coordinated myonuclear molecular landscape during muscle loading that coincides with an acute and rapid reduction of oxidative metabolism. This response may favor a biosynthesis-oriented metabolic program that supports rapid hypertrophic growth.","journal":"Function","year":2023,"id":333518,"datarank":0.5533041241157094,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"self_citation_contribution":0.47670807455219194,"citation_network_contribution":0.0765960495635175,"self_endowment_contribution":0.47670807455219194,"citer_contribution":0.0765960495635175,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":5,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9449,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE245715"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":628043,"name":"Nicholas T. Thomas","orcid":"0000-0003-4578-8463","position":1,"is_corresponding":false},{"id":1061295,"name":"Mariah McCashland","orcid":null,"position":2,"is_corresponding":false},{"id":286541,"name":"Ivan J. Vechetti","orcid":"0000-0003-1024-1011","position":3,"is_corresponding":false},{"id":1060886,"name":"Sebastian Edman","orcid":"0000-0003-2921-833X","position":4,"is_corresponding":false},{"id":632658,"name":"Johanna T. Lanner","orcid":"0000-0002-1222-9473","position":5,"is_corresponding":false},{"id":286542,"name":"Vandré C. Figueiredo","orcid":"0000-0002-3012-7905","position":6,"is_corresponding":false},{"id":286546,"name":"Christopher S. Fry","orcid":"0000-0002-4207-6594","position":7,"is_corresponding":false},{"id":282076,"name":"John J. McCarthy","orcid":"0000-0003-4522-5601","position":8,"is_corresponding":false},{"id":142302,"name":"Yuan Wen","orcid":null,"position":9,"is_corresponding":false},{"id":282073,"name":"Kevin A. Murach","orcid":"0000-0003-2783-7137","position":10,"is_corresponding":false},{"id":323869,"name":"Ferdinand von Walden","orcid":"0000-0003-1134-2252","position":11,"is_corresponding":false},{"id":669282,"name":"Ahmed Ismaeel","orcid":"0000-0003-4025-3945","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-19T01:09:39.719497Z","pmid":"38020067","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}