{"doi":"10.1093/femsle/fnw104","title":"The ferrichrome receptor A as a new target for<i>Pseudomonas aeruginosa</i>virulence attenuation","abstract":null,"journal":"FEMS Microbiology Letters","year":2016,"id":598561,"datarank":0.4335557636844247,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.0,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1533827,"name":"Kang-Mu Lee","orcid":null,"position":1,"is_corresponding":false},{"id":1533828,"name":"Junhyeok Go","orcid":null,"position":2,"is_corresponding":false},{"id":1533829,"name":"Jae-Chan Ryu","orcid":null,"position":3,"is_corresponding":false},{"id":317471,"name":"Ji-Hwan Ryu","orcid":"0000-0001-6969-7465","position":4,"is_corresponding":false},{"id":537986,"name":"Sang Sun Yoon","orcid":"0000-0003-2979-365X","position":5,"is_corresponding":false},{"id":575624,"name":"Keehoon Lee","orcid":"0000-0002-1751-3043","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The ferrichrome receptor A as a new target for<i>Pseudomonas aeruginosa</i>virulence attenuation","abstract":"Pseudomonas aeruginosa is an opportunistic pathogen, known to develop robust biofilms. Its biofilm development increases when antibiotics are presented at subminimal inhibitory concentrations (MICs) for reasons that remain unclear. In order to identify genes that affect biofilm development under such a sublethal antibiotic stress condition, we screened a transposon (Tn) mutant library of PAO1, a prototype P. aeruginosa strain. Among ∼5000 mutants, a fiuA gene mutant was verified to form very defective biofilms in the presence of sub-MIC carbenicillin. The fiuA gene encodes ferrichrome receptor A, involved in the iron acquisition process. Of note, biofilm formation was not decreased in the ΔpchΔpvd mutant defective in the production of pyochelin and pyoverdine, two well-characterized P. aeruginosa siderophore molecules. Moreover, ΔfiuA, a non-polar fiuA deletion mutant, produced a significantly decreased level of elastase, a major virulence determinant. Mouse airway infection experiments revealed that the mutant expressed significantly less pathogenicity. Our results suggest that the fiuA gene has pleiotropic functions that affect P. aeruginosa biofilm development and virulence. The targeting of FiuA could enable the attenuation of P. aeruginosa virulence and may be suitable for the development of a drug that specifically controls the virulence of this important pathogen.","is_dataset_classified":null,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27190289","pmcid":null,"openalex_id":"https://openalex.org/W2340149471","authors":[],"funders":[],"total_grants":0,"fwci":0.4809,"citation_percentile":0.68807496,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2019,"count":2},{"year":2020,"count":3},{"year":2021,"count":4},{"year":2022,"count":2},{"year":2023,"count":2},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"bronze","license":"cc-by-nc-nd","oa_locations":[{"url":"https://academic.oup.com/femsle/article-pdf/363/11/fnw104/23926546/fnw104.pdf","host_type":"journal"},{"url":"https://academic.oup.com/femsle/article-pdf/363/11/fnw104/23926546/fnw104.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/femsle/article-pdf/363/11/fnw104/10753338/fnw104.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/femsle/fnw104","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27190289","host_type":"repository"},{"url":"https://ir.ymlib.yonsei.ac.kr/handle/22282913/146856","host_type":"repository"},{"url":"http://femsle.oxfordjournals.org/cgi/content/short/363/11/fnw104","host_type":"repository"}],"fields_of_study":["Bacterial biofilms and quorum sensing","Antimicrobial Peptides and Activities","Bacterial Genetics and Biotechnology"],"mesh_terms":["Animals","Bacterial Outer Membrane Proteins","Carbenicillin","DNA Transposable Elements","Ferrichrome","Iron","Lung","Microbial Sensitivity Tests","Oligopeptides","Pancreatic Elastase","Phenols","Pseudomonas aeruginosa","Thiazoles","Gene Library","Sequence Deletion","Biofilms","Virulence Factors","Mice"],"keywords":["Virulence","Pseudomonas aeruginosa","Biofilm","Microbiology","Pyoverdine","Mutant","Biology","Ferrichrome","Siderophore","Pathogen","Swarming motility","Pyocyanin","Gene","Bacteria","Quorum sensing","Bacterial outer membrane","Genetics","Escherichia coli","Ferrichrome Receptor A"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T15:56:42.654369Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}