{"doi":"10.1093/emboj/cdg170","title":"Ribosomal protein S15 represses its own translation via adaptation of an rRNA‐like fold within its mRNA","abstract":null,"journal":"The EMBO Journal","year":2003,"id":48027,"datarank":2.9309535682227907,"base_score":4.1588830833596715,"endowment":4.1588830833596715,"self_citation_contribution":0.6238324625039509,"citation_network_contribution":2.30712110571884,"self_endowment_contribution":0.6238324625039509,"citer_contribution":2.30712110571884,"corpus_percentile":null,"corpus_rank":null,"citation_count":63,"citer_count":56,"citers_with_citation_signal":53,"citers_with_endowment":53,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":225004,"name":"Ann Polonskaia","orcid":null,"position":1,"is_corresponding":false},{"id":218864,"name":"Bernard Ehresmann","orcid":null,"position":2,"is_corresponding":false},{"id":138181,"name":"Chantal Ehresmann","orcid":null,"position":3,"is_corresponding":false},{"id":225005,"name":"Dinshaw J Patel","orcid":null,"position":4,"is_corresponding":false},{"id":138180,"name":"Alexander Serganov","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Ribosomal protein S15 represses its own translation via adaptation of an rRNA‐like fold within its mRNA","abstract":"The 16S rRNA-binding ribosomal protein S15 is a key component in the assembly of the small ribosomal subunit in bacteria. We have shown that S15 from the extreme thermophile Thermus thermophilus represses the translation of its own mRNA in vitro, by interacting with the leader segment of its mRNA. The S15 mRNA-binding site was characterized by footprinting experiments, deletion analysis and site-directed mutagenesis. S15 binding triggers a conformational rearrangement of its mRNA into a fold that mimics the conserved three-way junction of the S15 rRNA-binding site. This conformational change masks the ribosome entry site, as demonstrated by direct competition between the ribosomal subunit and S15 for mRNA binding. A comparison of the T.thermophilus and Escherichia coli regulation systems reveals that the two regulatory mRNA targets do not share any similarity and that the mechanisms of translational inhibition are different. Our results highlight an astonishing plasticity of mRNA in its ability to adapt to evolutionary constraints, that contrasts with the extreme conservation of the rRNA-binding site.","is_dataset_classified":null,"base_score":4.1588830833596715,"endowment":4.1588830833596715,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12682022","pmcid":"PMC154462","openalex_id":"https://openalex.org/W2053649551","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA46778","title":null},{"funder_name":"National Institutes of Health","grant_id":"2R01CA046778-06","title":"ANTITUMOR DRUG-DNA COMPLEXES IN SOLUTION"}],"total_grants":2,"fwci":1.9506,"citation_percentile":0.85634361,"influential_citations":3,"citation_trend":[{"year":2013,"count":5},{"year":2014,"count":3},{"year":2015,"count":3},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2018,"count":4},{"year":2020,"count":3},{"year":2021,"count":5},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":3}],"oa_status":"bronze","license":"Springer TDM","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1093/emboj/cdg170","host_type":"journal"},{"url":"https://europepmc.org/articles/pmc154462?pdf=render","host_type":"GREEN"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1093/emboj/cdg170","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1093%2Femboj%2Fcdg170","host_type":"publisher"},{"url":"https://link.springer.com/content/pdf/10.1093/emboj/cdg170.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1093/emboj/cdg170/fulltext.html","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full/10.1093/emboj/cdg170","host_type":"publisher"},{"url":"https://doi.org/10.1093/emboj/cdg170","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12682022","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/154462","host_type":"repository"},{"url":"https://dx.doi.org/10.1093/emboj/cdg170","host_type":""}],"fields_of_study":["RNA and protein synthesis mechanisms","Bacterial Genetics and Biotechnology","RNA modifications and cancer","Biology","Medicine","0301 basic medicine","0303 health sciences","03 medical and health sciences","Bacterial Proteins","Binding Sites","Nucleic Acid Conformation","Protein Binding","Protein Biosynthesis","Protein Footprinting","RNA, Messenger","Repressor Proteins","Ribosomal Proteins","Ribosomes","Thermus thermophilus"],"mesh_terms":["Bacterial Proteins","Binding Sites","Nucleic Acid Conformation","Protein Binding","Repressor Proteins","Ribosomal Proteins","Ribosomes","RNA, Messenger","Protein Biosynthesis","Thermus thermophilus","Protein Footprinting"],"keywords":["Biology","Thermus thermophilus","30S","Ribosomal protein","Ribosome","Ribosomal binding site","Ribosomal RNA","Translational frameshift","Messenger RNA","Eukaryotic Small Ribosomal Subunit","Eukaryotic Ribosome","A-site","23S ribosomal RNA","Translation (biology)","Genetics","Binding site","Cell biology","RNA","Escherichia coli","Gene","Ribosomal Proteins","Binding Sites","Repressor Proteins","Bacterial Proteins","Protein Biosynthesis","Nucleic Acid Conformation","Protein Footprinting","RNA, Messenger","Ribosomes","Protein Binding"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"},{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-18T20:23:01.685949Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}