{"doi":"10.1093/emboj/20.24.7271","title":"A novel transferable nuclear export signal mediates CRM1‐independent nucleocytoplasmic shuttling of the human cytomegalovirus transactivator protein pUL69","abstract":null,"journal":"The EMBO Journal","year":2001,"id":679897,"datarank":0.6766289259775276,"base_score":4.51085950651685,"endowment":4.51085950651685,"self_citation_contribution":0.6766289259775276,"citation_network_contribution":0.0,"self_endowment_contribution":0.6766289259775276,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":90,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1776428,"name":"Olaf Rosorius","orcid":null,"position":1,"is_corresponding":false},{"id":1776429,"name":"Erik Trommer","orcid":null,"position":2,"is_corresponding":false},{"id":851473,"name":"Thomas Stamminger","orcid":"0000-0001-9878-3119","position":3,"is_corresponding":false},{"id":779867,"name":"Peter Lischka","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A novel transferable nuclear export signal mediates CRM1‐independent nucleocytoplasmic shuttling of the human cytomegalovirus transactivator protein pUL69","abstract":"The best studied nuclear export processes are mediated by classical leucine-rich nuclear export signals that specify recognition by the CRM1 export receptor. However, details concerning alternative nuclear export signals and pathways are beginning to emerge. Within the family of Herpesviridae, a set of homologous regulatory proteins that are exemplified by the ICP27 of herpes simplex virus were described recently as nucleocytoplasmic shuttling proteins. Here we report that pUL69 of the beta-herpesvirus human cytomegalovirus is a nuclear protein that is able to shuttle between the nucleus and the cytoplasm independently of virus-encoded cofactors. In contrast to proteins containing a leucine-rich export signal, the shuttling activity of pUL69 was not affected by leptomycin B, indicating that pUL69 trafficking is not mediated by the export receptor CRM1. Importantly, we identified and characterized a novel type of transferable, leptomycin B-insensitive export signal that is distinct from other export signals described previously and is required for pUL69-mediated activation of gene expression. These data suggest that pUL69 is exported via a novel nuclear export pathway, based on a so far unique nuclear export signal of 28 amino acids.","is_dataset_classified":null,"base_score":4.51085950651685,"endowment":4.51085950651685,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11743003","pmcid":"PMC125785","openalex_id":"https://openalex.org/W1983994417","authors":[],"funders":[],"total_grants":0,"fwci":1.3427,"citation_percentile":0.79284676,"influential_citations":0,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":1},{"year":2014,"count":6},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2023,"count":2},{"year":2025,"count":3}],"oa_status":"green","license":"https://www.springer.com/tdm","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/125785","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/125785","host_type":"repository"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1093%2Femboj%2F20.24.7271","host_type":"publisher"},{"url":"https://link.springer.com/content/pdf/10.1093/emboj/20.24.7271.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1093/emboj/20.24.7271/fulltext.html","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full/10.1093/emboj/20.24.7271","host_type":"publisher"},{"url":"https://doi.org/10.1093/emboj/20.24.7271","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11743003","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC125785","host_type":"repository"}],"fields_of_study":["Nuclear Structure and Function","RNA regulation and disease","RNA Research and Splicing","Active Transport, Cell Nucleus","Amino Acid Sequence","Base Sequence","Cell Line","Cell Nucleus","Cytomegalovirus","Cytoplasm","Humans","Karyopherins","Microinjections","Molecular Sequence Data","Mutagenesis","Oligodeoxyribonucleotides","Protein Transport","Receptors, Cytoplasmic and Nuclear","Sequence Homology, Amino Acid","Trans-Activators","Transcriptional Activation","Viral Proteins","Exportin 1 Protein"],"mesh_terms":["Exportin 1 Protein","Amino Acid Sequence","Base Sequence","Cell Line","Cell Nucleus","Cytomegalovirus","Cytoplasm","Humans","Microinjections","Molecular Sequence Data","Oligodeoxyribonucleotides","Viral Proteins","Transcriptional Activation","Trans-Activators","Mutagenesis","Sequence Homology, Amino Acid","Receptors, Cytoplasmic and Nuclear","Protein Transport","Active Transport, Cell Nucleus","Karyopherins"],"keywords":["Biology","Transactivation","Nuclear export signal","Nuclear transport","Cell biology","Nuclear protein","Cell nucleus","Genetics","Virology","Nucleus","Gene","Gene expression","Transcription factor"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Partnerships for the goals"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T13:59:29.079423Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}