{"doi":"10.1093/cvr/cvaa312","title":"Selectin-targeting glycosaminoglycan-peptide conjugate limits neutrophil-mediated cardiac reperfusion injury","abstract":"AIMS: One of the hallmarks of myocardial infarction (MI) is excessive inflammation. During an inflammatory insult, damaged endothelial cells shed their glycocalyx, a carbohydrate-rich layer on the cell surface which provides a regulatory interface to immune cell adhesion. Selectin-mediated neutrophilia occurs as a result of endothelial injury and inflammation. We recently designed a novel selectin-targeting glycocalyx mimetic (termed DS-IkL) capable of binding inflamed endothelial cells. This study examines the capacity of DS-IkL to limit neutrophil binding and platelet activation on inflamed endothelial cells, as well as the cardioprotective effects of DS-IkL after acute myocardial infarction. METHODS AND RESULTS: In vitro, DS-IkL diminished neutrophil interactions with both recombinant selectin and inflamed endothelial cells, and limited platelet activation on inflamed endothelial cells. Our data demonstrated that DS-IkL localized to regions of vascular inflammation in vivo after 45 min of left anterior descending coronary artery ligation-induced MI. Further, findings from this study show DS-IkL treatment had short- and long-term cardioprotective effects after ischaemia/reperfusion of the left anterior descending coronary artery. Mice treated with DS-IkL immediately after ischaemia/reperfusion and 24 h later exhibited reduced neutrophil extravasation, macrophage accumulation, fibroblast and endothelial cell proliferation, and fibrosis compared to saline controls. CONCLUSIONS: Our findings suggest that DS-IkL has great therapeutic potential after MI by limiting reperfusion injury induced by the immune response.","journal":"Cardiovascular Research","year":2020,"id":78311,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":310949,"name":"Phung N. Thai","orcid":"0000-0001-7344-150X","position":1,"is_corresponding":false},{"id":252442,"name":"Harkanwalpreet Sodhi","orcid":"0000-0003-4614-503X","position":2,"is_corresponding":false},{"id":310944,"name":"Lu Ren","orcid":"0000-0002-9731-8562","position":3,"is_corresponding":false},{"id":355956,"name":"Padmini Sirish","orcid":"0000-0002-9216-279X","position":4,"is_corresponding":false},{"id":401355,"name":"Carol Nader","orcid":null,"position":5,"is_corresponding":false},{"id":401354,"name":"Valeriy Timofeyev","orcid":null,"position":6,"is_corresponding":false},{"id":310947,"name":"James Overton","orcid":"0000-0002-6527-1763","position":7,"is_corresponding":false},{"id":408263,"name":"Xiaocen Li","orcid":null,"position":8,"is_corresponding":false},{"id":237448,"name":"Kit S. Lam","orcid":"0000-0002-3076-6969","position":9,"is_corresponding":false},{"id":310948,"name":"Nipavan Chiamvimonvat","orcid":"0000-0001-9499-8817","position":10,"is_corresponding":false},{"id":252443,"name":"Alyssa Panitch","orcid":"0000-0002-0360-1480","position":11,"is_corresponding":false},{"id":407409,"name":"Tima Dehghani","orcid":"0000-0001-7563-2668","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-18T21:49:40.769098Z","pmid":"33125066","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}