{"doi":"10.1093/cvr/cvaa187","title":"Class switching and high-affinity immunoglobulin G production by B cells is dispensable for the development of hypertension in mice","abstract":"AIMS: Elevated serum immunoglobulins have been associated with experimental and human hypertension for decades but whether immunoglobulins and B cells play a causal role in hypertension pathology is unclear. In this study, we sought to determine the role of B cells and high-affinity class-switched immunoglobulins on hypertension and hypertensive end-organ damage to determine if they might represent viable therapeutic targets for this disease. METHODS AND RESULTS: We purified serum immunoglobulin G (IgG) from mice exposed to vehicle or angiotensin (Ang) II to induce hypertension and adoptively transferred these to wild type (WT) recipient mice receiving a subpressor dose of Ang II. We found that transfer of IgG from hypertensive animals does not affect blood pressure, endothelial function, renal inflammation, albuminuria, or T cell-derived cytokine production compared with transfer of IgG from vehicle infused animals. As an alternative approach to investigate the role of high-affinity, class-switched immunoglobulins, we studied mice with genetic deletion of activation-induced deaminase (Aicda-/-). These mice have elevated levels of IgM but virtual absence of class-switched immunoglobulins such as IgG subclasses and IgA. Neither male nor female Aicda-/- mice were protected from Ang II-induced hypertension and renal/vascular damage. To determine if IgM or non-immunoglobulin-dependent innate functions of B cells play a role in hypertension, we studied mice with severe global B-cell deficiency due to deletion of the membrane exon of the IgM heavy chain (µMT-/-). µMT-/- mice were also not protected from hypertension or end-organ damage induced by Ang II infusion or deoxycorticosterone acetate-salt treatment. CONCLUSIONS: These results suggest that B cells and serum immunoglobulins do not play a causal role in hypertension pathology.","journal":"Cardiovascular Research","year":2020,"id":76418,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9576,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":398250,"name":"Bethany L. Dale","orcid":"0000-0003-4984-5517","position":1,"is_corresponding":false},{"id":325637,"name":"Matthew R. Alexander","orcid":"0000-0003-4739-7204","position":2,"is_corresponding":false},{"id":225955,"name":"Liang Xiao","orcid":"0000-0003-0312-7149","position":3,"is_corresponding":false},{"id":298183,"name":"Mingfang Ao","orcid":"0000-0002-3639-8546","position":4,"is_corresponding":false},{"id":398251,"name":"Arvind K. Pandey","orcid":"0000-0001-7890-4665","position":5,"is_corresponding":false},{"id":16586,"name":"C. Duncan Smart","orcid":"0000-0003-0391-3916","position":6,"is_corresponding":false},{"id":398252,"name":"Gwendolyn K. Davis","orcid":"0000-0001-6547-8644","position":7,"is_corresponding":false},{"id":16598,"name":"Meena S. Madhur","orcid":"0000-0002-0407-634X","position":8,"is_corresponding":false},{"id":398249,"name":"Yuhan Chen","orcid":"0000-0002-2950-2675","position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":null,"created_at":"2026-07-18T21:47:46.469293Z","pmid":"32609312","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}