{"doi":"10.1093/clinchem/hvaf030","title":"Correlations Within and Between Highly Multiplexed Proteomic Assays of Human Plasma","abstract":"INTRODUCTION: The number of assays on proteomic platforms has grown rapidly. The leading platforms, SomaScan and Olink, have strengths and limitations. Comparisons of precision on the latest platforms-SomaScan 11k and Olink Explore HT-have not yet been established. METHODS: Among 102 participants in the Atherosclerosis Risk in Communities Study (mean age 74 years, 53% women, 47% Black), we used split plasma samples to measure platform precision. CV and Spearman correlations were calculated for each assay. Cross-platform agreement was assessed for overlapping proteins. RESULTS: SomaScan 11k demonstrated a median correlation of 0.85 for the 10 778 assays and a median CV of 6.8%, similar precision to earlier versions. The 5420 assays on Olink Explore HT exhibited a median correlation of 0.65 and median CV of 35.7%, which was higher than observed in its predecessors (e.g., 19.8% for Olink Explore 3072). Precision of Olink assays was inversely correlated with the percentage of samples above the limit of detection (LOD) (r = -0.77). Upon replacing Olink values below the LOD with values half the LOD, the median correlation for Olink assays measured in duplicate increased to 0.79; the median CV decreased to 13.3%. The distribution of between-platform correlations for the 4443 overlapping proteins had peaks at r approximately 0 and at r approximately 0.8. One-tenth of the protein pairs had cross-platform correlations r ≥ 0.8. CONCLUSIONS: Precision of these 2 proteomics platforms in human plasma has diverged as the coverage has increased. These results highlight the need for careful consideration in platform selection based on specific research requirements.","journal":"Clinical Chemistry","year":2025,"id":510458,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8921,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":624479,"name":"Jingsha Chen","orcid":null,"position":1,"is_corresponding":false},{"id":233725,"name":"Christie M. Ballantyne","orcid":"0000-0002-6432-1730","position":2,"is_corresponding":false},{"id":233724,"name":"Ron C. Hoogeveen","orcid":"0000-0003-2399-4653","position":3,"is_corresponding":false},{"id":118530,"name":"Eric Boerwinkle","orcid":null,"position":4,"is_corresponding":false},{"id":538012,"name":"Bing Yu","orcid":"0000-0001-6605-6742","position":5,"is_corresponding":false},{"id":34922,"name":"Keenan A. Walker","orcid":"0000-0002-5989-9853","position":6,"is_corresponding":false},{"id":334102,"name":"Pascal Schlosser","orcid":"0000-0002-8460-0462","position":7,"is_corresponding":false},{"id":405218,"name":"Elizabeth Selvin","orcid":"0000-0002-3539-2070","position":8,"is_corresponding":false},{"id":13338,"name":"Nilanjan Chatterjee","orcid":"0000-0002-9060-008X","position":9,"is_corresponding":false},{"id":250083,"name":"David Couper","orcid":"0000-0002-4313-9235","position":10,"is_corresponding":false},{"id":73169,"name":"Morgan E. Grams","orcid":"0000-0002-4430-6023","position":11,"is_corresponding":false},{"id":23021,"name":"Josef Coresh","orcid":"0000-0002-4598-0669","position":12,"is_corresponding":false},{"id":441182,"name":"Mary R. Rooney","orcid":"0000-0002-5607-4848","position":0,"is_corresponding":true}],"reference_count":14,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:47:36.538276Z","pmid":"40172053","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}