{"doi":"10.1093/clinchem/28.3.488","title":"Radioimmunoassay for somatomedin C: comparison with radioreceptor assay in patients with growth-hormone disorders, hypothyroidism, and renal failure.","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>We raised an antiserum (Tr4) in rabbits against a basic somatomedin C-like peptide preparation. Using high-immunoreactivity somatomedin C tracer, we compared the performance of radioimmunoassays in which we used the Tr4 antiserum and a well-characterized somatomedin C antiserum distributed by the National Pituitary Agency (NPA) with that of the human placental-membrane somatomedin radioreceptor assay (RRA). In their cross reactivity toward various somatomedin-like and unrelated peptides, the two radioimmunoassay methods were almost identical, although NPA antiserum, with about fourfold higher titer than Tr4 antiserum, showed a slightly greater sensitivity for most peptides tested. Radioimmunoassay of acid-ethanol-extracted plasma samples from normal persons and acromegalic, hypopituitary, hypothyroid, and renal-failure patients revealed no analytical differences between the antisera (for 122 samples, r = 0.979 between methods). Somatomedin values for acromegalic and hypopituitary samples showed no overlap with normals. Values for hypothyroid and pre-dialysis renal-failure samples were significantly lower than normal. By comparison, the RRA showed greater cross reactivity toward some somatomedin-like peptides and gave significantly lower values than radioimmunoassay for acromegalic and hypothyroid plasma extracts, and significantly higher value for hypopituitary and renal-failure samples. We conclude that the radioimmunoassay methods clearly are of greater diagnostic value than RRA for clinical somatomedin measurement.</jats:p>","journal":"Clinical Chemistry","year":1982,"id":15670,"datarank":10.070545529365535,"base_score":4.890349128221754,"endowment":4.890349128221754,"self_citation_contribution":0.7335523692332632,"citation_network_contribution":9.336993160132272,"self_endowment_contribution":0.7335523692332632,"citer_contribution":9.336993160132272,"corpus_percentile":null,"corpus_rank":null,"citation_count":132,"citer_count":103,"citers_with_citation_signal":99,"citers_with_endowment":99,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":118735,"name":"A S Brown","orcid":null,"position":1,"is_corresponding":false},{"id":118736,"name":"J R Turtle","orcid":null,"position":2,"is_corresponding":false},{"id":118733,"name":"R C Baxter","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.890349128221754,"endowment":4.890349128221754,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7199977","pmcid":null,"openalex_id":"https://openalex.org/W1931638239","authors":[],"funders":[],"total_grants":0,"fwci":2.9282,"citation_percentile":0.9071028,"influential_citations":1,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2015,"count":1},{"year":2018,"count":1},{"year":2020,"count":2},{"year":2022,"count":1}],"oa_status":"closed","license":"https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model","oa_locations":[{"url":"http://academic.oup.com/clinchem/article-pdf/28/3/488/32798861/clinchem0488.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/clinchem/28.3.488","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7199977","host_type":"repository"}],"fields_of_study":["Radiopharmaceutical Chemistry and Applications","Chemistry","Medicine"],"mesh_terms":["Acromegaly","Humans","Hypopituitarism","Hypothyroidism","Insulin-Like Growth Factor I","Kidney Diseases","Quality Control","Radioimmunoassay","Radioligand Assay","Regression Analysis","Somatomedins"],"keywords":["Radioimmunoassay","Somatomedin","Antiserum","Endocrinology","Internal medicine","Titer","Chemistry","Hormone","Growth hormone","Medicine","Antibody","Immunology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T18:33:26.243469Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}