{"doi":"10.1093/clinchem/20.4.470","title":"Enzymatic Determination of Total Serum Cholesterol","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-one with the simultaneous production of hydrogen peroxide, which oxidatively couples with 4-aminoantipyrine and phenol in the presence of peroxidase to yield a chromogen with maximum absorption at 500 nm. The method is reproducible, and the results correlate well with those obtained by automated Liebermann—Burchard procedures (AA-2 and SMA 12/60) and the method of Abell et al. The present method affords better specificity than those previously reported and has excellent precision.</jats:p>","journal":"Clinical Chemistry","year":1974,"id":9532,"datarank":32.158074652402945,"base_score":9.120087382998621,"endowment":9.120087382998621,"self_citation_contribution":1.3680131074497934,"citation_network_contribution":30.79006154495315,"self_endowment_contribution":1.3680131074497934,"citer_contribution":30.79006154495315,"corpus_percentile":98.8,"corpus_rank":1740,"citation_count":9136,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"1974-04-01","authors":[{"id":79911,"name":"Lucy S Poon","orcid":null,"position":1,"is_corresponding":false},{"id":79912,"name":"Cicely S G Chan","orcid":null,"position":2,"is_corresponding":false},{"id":79913,"name":"W. Richmond","orcid":null,"position":3,"is_corresponding":false},{"id":79914,"name":"Paul C Fu","orcid":null,"position":4,"is_corresponding":false},{"id":79915,"name":"Paul Fu","orcid":"0000-0001-5772-047X","position":5,"is_corresponding":false},{"id":79910,"name":"Charles C. Allain","orcid":null,"position":0,"is_corresponding":true}],"reference_count":12,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}