{"doi":"10.1093/cid/ciw427","title":"Evaluation of the C6 Lyme Enzyme Immunoassay for the Diagnosis of Lyme Disease in Children and Adolescents","abstract":null,"journal":"Clinical Infectious Diseases","year":2016,"id":602655,"datarank":0.5495342469194471,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"self_citation_contribution":0.5495342469194471,"citation_network_contribution":0.0,"self_endowment_contribution":0.5495342469194471,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":108260,"name":"John A. Branda","orcid":"0000-0002-6929-1219","position":1,"is_corresponding":false},{"id":276829,"name":"Alexander J. McAdam","orcid":"0009-0005-6922-4760","position":2,"is_corresponding":false},{"id":1545716,"name":"Louis Vernacchio","orcid":null,"position":3,"is_corresponding":false},{"id":1377550,"name":"Caroline D. Gordon","orcid":null,"position":4,"is_corresponding":false},{"id":1545717,"name":"Catherine R. Gordon","orcid":null,"position":5,"is_corresponding":false},{"id":302514,"name":"Lise E. Nigrovic","orcid":"0000-0002-6369-3997","position":6,"is_corresponding":false},{"id":481527,"name":"Susan C. Lipsett","orcid":"0000-0002-4758-6905","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Evaluation of the C6 Lyme Enzyme Immunoassay for the Diagnosis of Lyme Disease in Children and Adolescents","abstract":"BACKGROUND: The commercially-available C6 Lyme enzyme immunoassay (EIA) has been approved to replace the standard whole-cell sonicate EIA as a first-tier test for the diagnosis of Lyme disease and has been suggested as a stand-alone diagnostic. However, the C6 EIA has not been extensively studied in pediatric patients undergoing evaluation for Lyme disease. METHODS: We collected discarded serum samples from children and adolescents (aged ≤21 years) undergoing conventional 2-tiered testing for Lyme disease at a single hospital-based clinical laboratory located in an area endemic for Lyme disease. We performed a C6 EIA on all collected specimens, followed by a supplemental immunoblot if the C6 EIA result was positive but the whole-cell sonicate EIA result was negative. We defined a case of Lyme disease as either a clinician-diagnosed erythema migrans lesion or a positive standard 2-tiered serologic result in a patient with symptoms compatible with Lyme disease. We then compared the performance of the C6 EIA alone and as a first-tier test followed by immunoblot, with that of standard 2-tiered serology for the diagnosis of Lyme disease. RESULTS: Of the 944 specimens collected, 114 (12%) were from patients with Lyme disease. The C6 EIA alone had sensitivity similar to that of standard 2-tiered testing (79.8% vs 81.6% for standard 2-tiered testing; P = .71) with slightly lower specificity (94.2% vs 98.8% 2; P < .002). Addition of a supplemental immunoblot improved the specificity of the C6 EIA to 98.6%. CONCLUSIONS: For children and adolescents undergoing evaluation for Lyme disease, the C6 EIA could guide initial clinical decision making, although a supplemental immunoblot should still be performed.","is_dataset_classified":null,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27358358","pmcid":"PMC5019286","openalex_id":"https://openalex.org/W2468702102","authors":[],"funders":[{"funder_name":"NCATS NIH HHS","grant_id":"UL1 TR001102","title":null},{"funder_name":"Harvard Medical School","grant_id":"","title":null},{"funder_name":"National Institutes of Health","grant_id":"","title":null},{"funder_name":"Boston Children&apos;s Hospital","grant_id":"","title":null},{"funder_name":"Harvard Catalyst Early Clinical Data","grant_id":"","title":null},{"funder_name":"Research Faculty Council","grant_id":"","title":null}],"total_grants":6,"fwci":5.1927,"citation_percentile":0.95805987,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2017,"count":5},{"year":2018,"count":10},{"year":2019,"count":4},{"year":2020,"count":5},{"year":2021,"count":3},{"year":2022,"count":2},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":4}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/cid/article-pdf/63/7/922/7081493/ciw427.pdf","host_type":"journal"},{"url":"https://academic.oup.com/cid/article-pdf/63/7/922/7081493/ciw427.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/cid/article-pdf/63/7/922/7081493/ciw427.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/cid/ciw427","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27358358","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5019286","host_type":"repository"},{"url":"http://cid.oxfordjournals.org/cgi/content/short/63/7/922","host_type":"repository"}],"fields_of_study":["Vector-borne infectious diseases","Parasites and Host Interactions","Complement system in diseases","Adolescent","Antibodies, Bacterial","Borrelia burgdorferi","Child","Cross-Sectional Studies","Female","Humans","Immunoenzyme Techniques","Lyme Disease","Male","Sensitivity and Specificity"],"mesh_terms":["Adolescent","Antibodies, Bacterial","Child","Cross-Sectional Studies","Female","Humans","Immunoenzyme Techniques","Lyme Disease","Male","Sensitivity and Specificity","Borrelia burgdorferi"],"keywords":["Lyme disease","Medicine","LYME","Serology","Erythema migrans","Immunoassay","Internal medicine","Immunology","Borrelia burgdorferi","Antibody","Lyme borreliosis","Pediatrics","C6","Lyme Disease Diagnostics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Peace, Justice and strong institutions"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T19:56:26.718203Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}