{"doi":"10.1093/chemse/28.5.415","title":"Inward currents and increases in cytosolic Ca2+ concentration induced by cyclic ADP-ribose in turtle olfactory receptor cells","abstract":null,"journal":"Chemical Senses","year":2003,"id":662654,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1729969,"name":"K. Sekimoto","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Inward currents and increases in cytosolic Ca2+ concentration induced by cyclic ADP-ribose in turtle olfactory receptor cells","abstract":"In olfactory receptor cells, it is well established that cyclic AMP (cAMP) and inositol-1,4,5-trisphosphate (IP(3)) act as second messengers during odor responses. In previous studies, we have shown that cAMP-increasing odorants induce odor responses even after complete desensitization of the cAMP-mediated pathway. These results suggest that at least one cAMP-independent pathway contributes to the generation of odor responses. In an attempt to identify a novel second messenger, we investigated the possible role of cyclic ADP-ribose (cADPR) in olfactory transduction. Turtle olfactory receptor cells were isolated using an enzyme-free procedure and loaded with fura-2/AM. The cells responded to dialysis with cADPR with an inward current and an increase of the intracellular Ca(2+) concentration, [Ca(2+)](i). Flooding of cells with 100 microM cADPR from the pipette also induced an inward current without changes in [Ca(2+)](i) in Na(+)-containing and Ca(2+)-free Ringer solution. In an Na(+)-free and Ca(2+)-containing Ringer solution, cADPR induced only a small inward current with a concomitant increase in [Ca(2+)](i). Inward currents and increases in [Ca(2+)](i) induced by cADPR were completely inhibited by removal of both Na(+) and Ca(2+) from the outer solution. The experiments suggest that cADPR activates a cation channel at the plasma membrane, allowing inflow of Na(+) and Ca(2+) ions. The magnitudes of the inward current responses to cAMP-increasing odorants were greatly reduced by prior dialyses of a high concentration of cADPR or 8-bromo-cyclic ADP-ribose (8-Br-cADPR), an antagonist. It is possible that the cADPR-dependent pathway contributes to the generation of olfactory responses.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12826537","pmcid":null,"openalex_id":"https://openalex.org/W2144784232","authors":[],"funders":[],"total_grants":0,"fwci":0.1687,"citation_percentile":0.51958689,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/chemse/article-pdf/28/5/415/9754237/280415.pdf","host_type":"journal"},{"url":"https://academic.oup.com/chemse/article-pdf/28/5/415/9754237/280415.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/chemse/article-pdf/28/5/415/9754237/280415.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/chemse/28.5.415","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12826537","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.424.2308","host_type":""}],"fields_of_study":["Calcium signaling and nucleotide metabolism","Piperaceae Chemical and Biological Studies","Ion Channels and Receptors","Animals","Calcium","Cyclic ADP-Ribose","Cytosol","Dose-Response Relationship, Drug","Electric Conductivity","Olfactory Receptor Neurons","Turtles"],"mesh_terms":["Animals","Calcium","Cytosol","Dose-Response Relationship, Drug","Electric Conductivity","Turtles","Olfactory Receptor Neurons","Cyclic ADP-Ribose"],"keywords":["Cyclic ADP-ribose","Second messenger system","Olfactory Receptor Cell","Biophysics","Chemistry","Cyclase","Inositol trisphosphate","Cyclic nucleotide-gated ion channel","Intracellular","Cytosol","Receptor","Odor","Olfactory receptor","Calcium","Inositol","Cell biology","Biochemistry","Biology","Cyclic nucleotide","Enzyme","Nucleotide"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T16:46:19.693456Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}