{"doi":"10.1093/cercor/bhac275","title":"Biological constraints on stereotaxic targeting of functionally-defined cortical areas","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Understanding computational principles in hierarchically organized sensory systems requires functional parcellation of brain structures and their precise targeting for manipulations. Although brain atlases are widely used to infer area locations in the mouse neocortex, it has been unclear whether stereotaxic coordinates based on standardized brain morphology accurately represent functional domains in individual animals. Here, we used intrinsic signal imaging to evaluate the accuracy of area delineation in the atlas by mapping functionally-identified auditory cortices onto bregma-based stereotaxic coordinates. We found that auditory cortices in the brain atlas correlated poorly with the true complexity of functional area boundaries. Inter-animal variability in functional area locations predicted surprisingly high error rates in stereotaxic targeting with atlas coordinates. This variability was not simply attributed to brain sizes or suture irregularities but instead reflected differences in cortical geography across animals. Our data thus indicate that functional mapping in individual animals is essential for dissecting cortical area-specific roles with high precision.</jats:p>","journal":"Cerebral Cortex","year":2023,"id":687329,"datarank":0.48283137373023016,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"self_citation_contribution":0.48283137373023016,"citation_network_contribution":0.0,"self_endowment_contribution":0.48283137373023016,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":690675,"name":"Hiroaki Tsukano","orcid":"0000-0001-8511-7256","position":1,"is_corresponding":false},{"id":690674,"name":"Amber M. Kline","orcid":"0000-0001-6128-5044","position":2,"is_corresponding":false},{"id":888494,"name":"Koun Onodera","orcid":"0000-0002-4203-9865","position":3,"is_corresponding":false},{"id":597183,"name":"Hiroyuki Kato","orcid":"0000-0002-5748-3221","position":4,"is_corresponding":false},{"id":1795632,"name":"Divya P Narayanan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Biological constraints on stereotaxic targeting of functionally-defined cortical areas","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Understanding computational principles in hierarchically organized sensory systems requires functional parcellation of brain structures and their precise targeting for manipulations. Although brain atlases are widely used to infer area locations in the mouse neocortex, it has been unclear whether stereotaxic coordinates based on standardized brain morphology accurately represent functional domains in individual animals. Here, we used intrinsic signal imaging to evaluate the accuracy of area delineation in the atlas by mapping functionally-identified auditory cortices onto bregma-based stereotaxic coordinates. We found that auditory cortices in the brain atlas correlated poorly with the true complexity of functional area boundaries. Inter-animal variability in functional area locations predicted surprisingly high error rates in stereotaxic targeting with atlas coordinates. This variability was not simply attributed to brain sizes or suture irregularities but instead reflected differences in cortical geography across animals. Our data thus indicate that functional mapping in individual animals is essential for dissecting cortical area-specific roles with high precision.</jats:p>","is_dataset_classified":null,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35834935","pmcid":"PMC10016058","openalex_id":"https://openalex.org/W4285493706","authors":[],"funders":[{"funder_name":"National Institute on Deafness and Other Communication Disorders","grant_id":"R01DC017516","title":null},{"funder_name":"National Institute of Neurological Disorders and Stroke","grant_id":"F31-NS111849","title":null},{"funder_name":"National Institute of Neurological Disorders and Stroke","grant_id":"T32-NS007431","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"F31 NS111849","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"R01 NS128873","title":null},{"funder_name":"NINDS NIH 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