{"doi":"10.1093/cei/uxaf086","title":"IL-33 blockade attenuates vascular inflammation in a mouse model of Kawasaki disease vasculitis","abstract":"The immune mechanisms underlying Kawasaki disease (KD), a febrile systemic vasculitis in children, are poorly understood. Reports indicate elevated levels of circulating IL-33 in acute KD patients; however, if IL-33 contributes to the pathogenesis of KD vasculitis remains unclear. Here, we used the Lactobacillus casei cell wall extract (LCWE)-induced murine model of KD to determine the contribution of IL-33 to vasculitis development. We observed increased expression of Il33 transcripts and IL-33 protein in LCWE-induced cardiovascular lesions. Bone marrow chimera experiments suggest that IL-33 production by both hematopoietic and stromal cells is important for LCWE-induced KD vasculitis; however, single-cell RNA sequencing, spatial transcriptomics, and flow cytometric analysis revealed that stromal cells were the predominant sources of IL-33. Furthermore, immune cells infiltrating LCWE-induced cardiovascular lesions expressed Il1rl1 transcripts, coding for the IL-33 receptor ST2. In vitro stimulation of bone marrow-derived macrophages with IL-33 enhanced their production of IL-1b and TNF-α. In vivo blockade of IL-33, using either neutralizing IL-33 antibody or Il33-/- mice, effectively attenuated LCWE-induced cardiovascular inflammation. Our results indicate that IL-33 contributes to LCWE-induced vascular inflammation through redundant mechanisms across multiple immune cell subsets rather than a single population and highlight IL-33 as a potential therapeutic target.","journal":"Clinical & Experimental Immunology","year":2025,"id":549869,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9628,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1445254,"name":"B. Evan Ross","orcid":null,"position":1,"is_corresponding":false},{"id":302305,"name":"Prasant Kumar Jena","orcid":"0000-0002-6729-5881","position":2,"is_corresponding":false},{"id":873956,"name":"Asli E. Atici","orcid":"0000-0003-1546-7191","position":3,"is_corresponding":false},{"id":1445255,"name":"Angela C Gomez","orcid":null,"position":4,"is_corresponding":false},{"id":135665,"name":"Michael C Fishbein","orcid":null,"position":5,"is_corresponding":false},{"id":1445256,"name":"Emily Aubuchon","orcid":null,"position":6,"is_corresponding":false},{"id":51344,"name":"Youngho Lee","orcid":null,"position":7,"is_corresponding":false},{"id":540645,"name":"R. Lee","orcid":null,"position":8,"is_corresponding":false},{"id":287793,"name":"Elizabeth A. Jacobsen","orcid":"0000-0003-2956-753X","position":9,"is_corresponding":false},{"id":1445257,"name":"Waldiceu Verri Jr","orcid":null,"position":10,"is_corresponding":false},{"id":1445258,"name":"S. C. Chen","orcid":null,"position":11,"is_corresponding":false},{"id":315014,"name":"Timothy R. Crother","orcid":"0000-0001-8465-6127","position":12,"is_corresponding":false},{"id":231575,"name":"Moshe Arditi","orcid":"0000-0001-9042-2909","position":13,"is_corresponding":false},{"id":231571,"name":"Magali Noval Rivas","orcid":"0000-0001-5570-8928","position":14,"is_corresponding":false},{"id":1445253,"name":"Thacyana Teixeira de Carvalho","orcid":null,"position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-19T02:54:12.321988Z","pmid":"41533763","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}