{"doi":"10.1091/mbc.e24-10-0443","title":"Aβ impairs the LTP-related movement of endogenous CaMKII but not of exogenous GFP-CaMKII","abstract":"/calmodulin (CaM)-dependent protein kinase II (CaMKII). Notably, CaMKII inhibition rescues both CaMKII movement and LTP, indicating that CaMKII mediates both LTP and the Aβ-induced LTP impairment. Somewhat counterintuitively, we found here that overexpression of GFP-CaMKII also rescued the Aβ-induced impairment of CaMKII movement. For endogenous CaMKII, we confirmed that Aβ indeed induced impairment of movement, and that previous results with live-imaging approaches were not due to Aβ-induced dissociation of the CaMKII intrabody. For exogenous GFP-CaMKII, the effect did not depend on the expression level and was thus likely caused by the N-terminal GFP label. Surprisingly, placing the GFP label instead at the C-terminus (near the association domain) still allowed CaMKII holoenzyme formation and still protected from the Aβ-induced impairment of CaMKII movement. Thus, while our method allows replacing endogenous CaMKII with similar amounts of GFP-CaMKII, our results provide a rare example for GFP-CaMKII not recapitulating the function of endogenous CaMKII.","journal":"Molecular Biology of the Cell","year":2025,"id":552139,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9596,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1068432,"name":"Fan-Yi Chao","orcid":"0000-0001-7449-6748","position":1,"is_corresponding":false},{"id":1014847,"name":"Daphne Quang","orcid":"0000-0001-9064-6531","position":2,"is_corresponding":false},{"id":345713,"name":"Nicole L. Rumian","orcid":"0000-0002-4327-1930","position":3,"is_corresponding":false},{"id":1447993,"name":"Mason S. Kleinjan","orcid":"0000-0002-6148-4606","position":4,"is_corresponding":false},{"id":345715,"name":"Steven J. Coultrap","orcid":"0009-0005-7272-6358","position":5,"is_corresponding":false},{"id":345718,"name":"K. Ulrich Bayer","orcid":"0000-0003-0893-2200","position":6,"is_corresponding":false},{"id":416627,"name":"Carolyn Nicole Brown","orcid":"0000-0002-3612-1125","position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-19T02:54:29.318076Z","pmid":"40137857","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}