{"doi":"10.1091/mbc.e24-04-0189","title":"Big1 is a newly identified autophagy regulator that is critical for a fully functional V-ATPase","abstract":"The vacuolar-type H + -translocating ATPase (V-ATPase) is the major proton pump for intraorganellar acidification. Therefore, the integrity of the V-ATPase is closely associated with cellular homeostasis, and mutations in genes encoding V-ATPase components and assembly factors have been reported in certain types of diseases. For instance, the recurrent mutations of ATP6AP1, a gene encoding a V-ATPase accessory protein, have been associated with cancers and immunodeficiency. With the aim of studying V-ATPase–related mutations using the yeast model system, we report that Big1 is another homologue of ATP6AP1 in yeast cells, and we characterize the role of Big1 in maintaining a fully functional V-ATPase. In addition to its role in acidifying the vacuole or lysosome, our data support the concept that the V-ATPase may function as part of a signaling pathway to regulate macroautophagy/autophagy through a mechanism that is independent from Tor/MTOR.","journal":"Molecular Biology of the Cell","year":2024,"id":477509,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1315782,"name":"Ying Yang","orcid":"0009-0004-6879-0982","position":1,"is_corresponding":false},{"id":634540,"name":"Zhihai Zhang","orcid":"0000-0001-7870-1112","position":2,"is_corresponding":false},{"id":910059,"name":"Ruoxi Zhang","orcid":"0000-0003-2538-1877","position":3,"is_corresponding":false},{"id":257788,"name":"Xinxin Song","orcid":"0000-0003-3868-2644","position":4,"is_corresponding":false},{"id":1182390,"name":"Sami N. Malek","orcid":"0000-0001-7734-4387","position":5,"is_corresponding":false},{"id":103781,"name":"Daolin Tang","orcid":"0000-0002-1903-6180","position":6,"is_corresponding":false},{"id":71199,"name":"Daniel J. Klionsky","orcid":"0000-0002-7828-8118","position":7,"is_corresponding":false},{"id":267877,"name":"Yuchen Lei","orcid":"0000-0003-2112-4144","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:06:37.812633Z","pmid":"39259764","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}