{"doi":"10.1091/mbc.e24-02-0067","title":"Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation in <i>Drosophila</i> oocytes","abstract":"The reductional division of meiosis I requires the separation of chromosome pairs towards opposite poles. We have previously implicated the outer kinetochore protein SPC105R/KNL1 in driving meiosis I chromosome segregation through lateral attachments to microtubules and coorientation of sister centromeres. To identify the domains of SPC105R that are critical for meiotic chromosome segregation, an RNAi-resistant gene expression system was developed. We found that the SPC105R C-terminal domain (aa 1284-1960) is necessary and sufficient for recruiting NDC80 to the kinetochore and building the outer kinetochore. Furthermore, the C-terminal domain recruits BUBR1, which in turn recruits the cohesion protection proteins MEI-S332 and PP2A. Of the remaining 1283 amino acids, we found the first 473 are most important for meiosis. The first 123 amino acids of the N-terminal half of SPC105R contain the conserved SLRK and RISF motifs that are targets of PP1 and Aurora B kinase and are most important for regulating the stability of microtubule attachments and maintaining metaphase I arrest. The region between amino acids 124 and 473 are required for lateral microtubule attachments and biorientation of homologues, which are critical for accurate chromosome segregation in meiosis I.","journal":"Molecular Biology of the Cell","year":2024,"id":457798,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9529,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1082127,"name":"Neha Changela","orcid":null,"position":1,"is_corresponding":false},{"id":1283728,"name":"Lia Mahal","orcid":null,"position":2,"is_corresponding":false},{"id":587120,"name":"Janet K. Jang","orcid":"0009-0006-4112-4351","position":3,"is_corresponding":false},{"id":587587,"name":"Tyler Defosse","orcid":null,"position":4,"is_corresponding":false},{"id":587119,"name":"Lin-Ing Wang","orcid":"0000-0001-7916-9885","position":5,"is_corresponding":false},{"id":719117,"name":"Arunika Das","orcid":"0000-0001-7116-3489","position":6,"is_corresponding":false},{"id":719896,"name":"Joanatta G. Shapiro","orcid":null,"position":7,"is_corresponding":false},{"id":587122,"name":"Kim S. McKim","orcid":"0000-0002-8990-1168","position":8,"is_corresponding":false},{"id":1283309,"name":"Jay N. Joshi","orcid":"0000-0003-0267-1150","position":0,"is_corresponding":true}],"reference_count":89,"raw_metadata":null,"created_at":"2026-07-19T02:03:41.744499Z","pmid":"38865189","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}