{"doi":"10.1091/mbc.e23-10-0388","title":"Tools for live-cell imaging of cytoskeletal and nuclear behavior in the unconventional yeast, <i>Aureobasidium pullulans</i>","abstract":"Aureobasidium pullulans is a ubiquitous fungus with a wide variety of morphologies and growth modes including “typical” single-budding yeast, and interestingly, larger multinucleate yeast than can make multiple buds in a single cell cycle. The study of A. pullulans promises to uncover novel cell biology, but currently tools are lacking to achieve this goal. Here, we describe initial components of a cell biology toolkit for A. pullulans, which is used to express and image fluorescent probes for nuclei as well as components of the cytoskeleton. These tools allowed live-cell imaging of the multinucleate and multibudding cycles, revealing highly synchronous mitoses in multinucleate yeast that occur in a semiopen manner with an intact but permeable nuclear envelope. These findings open the door to using this ubiquitous polyextremotolerant fungus as a model for evolutionary cell biology.","journal":"Molecular Biology of the Cell","year":2024,"id":430976,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":816251,"name":"Alex W. Crocker","orcid":"0000-0003-1592-0777","position":1,"is_corresponding":false},{"id":1234790,"name":"Alec D’Alessandro","orcid":null,"position":2,"is_corresponding":false},{"id":1234356,"name":"Edgar M. Medina","orcid":"0000-0002-5518-5933","position":3,"is_corresponding":false},{"id":994874,"name":"Olivia Gorman","orcid":null,"position":4,"is_corresponding":false},{"id":1234357,"name":"Jessica McNeill","orcid":"0000-0003-4260-9706","position":5,"is_corresponding":false},{"id":5955,"name":"Amy S. Gladfelter","orcid":"0000-0002-2490-6945","position":6,"is_corresponding":false},{"id":525538,"name":"Daniel J. Lew","orcid":"0000-0001-7482-3585","position":7,"is_corresponding":false},{"id":1234355,"name":"Claudia A. Petrucco","orcid":"0000-0002-9025-1609","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T01:59:20.861374Z","pmid":"38446617","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}