{"doi":"10.1091/mbc.e23-07-0287","title":"The kinesin-5 tail and bipolar minifilament domains are the origin of its microtubule crosslinking and sliding activity","abstract":"Kinesin-5 crosslinks and slides apart microtubules to assemble, elongate, and maintain the mitotic spindle. Kinesin-5 is a tetramer, where two N-terminal motor domains are positioned at each end of the motor, and the coiled-coil stalk domains are organized into a tetrameric bundle through the bipolar assembly (BASS) domain. To dissect the function of the individual structural elements of the motor, we constructed a minimal kinesin-5 tetramer (mini-tetramer). We determined the x-ray structure of the extended, 34-nm BASS domain. Guided by these structural studies, we generated active bipolar kinesin-5 mini-tetramer motors from Drosophila melanogastor and human orthologues which are half the length of native kinesin-5. We then used these kinesin-5 mini-tetramers to examine the role of two unique structural adaptations of kinesin-5: 1) the length and flexibility of the tetramer, and 2) the C-terminal tails which interact with the motor domains to coordinate their ATPase activity. The C-terminal domain causes frequent pausing and clustering of kinesin-5. By comparing microtubule crosslinking and sliding by mini-tetramer and full-length kinesin-5, we find that both the length and flexibility of kinesin-5 and the C-terminal tails govern its ability to crosslink microtubules. Once crosslinked, stiffer mini-tetramers slide antiparallel microtubules more efficiently than full-length motors.","journal":"Molecular Biology of the Cell","year":2023,"id":362716,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":815286,"name":"Malina K. Iwanski","orcid":"0000-0002-4903-9796","position":1,"is_corresponding":false},{"id":326786,"name":"Ignas Gaska","orcid":"0009-0005-2569-0751","position":2,"is_corresponding":false},{"id":566263,"name":"Himanshu Pandey","orcid":"0000-0002-0629-7525","position":3,"is_corresponding":false},{"id":241647,"name":"Tatyana Bodrug","orcid":"0000-0001-9017-962X","position":4,"is_corresponding":false},{"id":326788,"name":"Sayaka Inagaki","orcid":"0000-0001-9064-0762","position":5,"is_corresponding":false},{"id":326787,"name":"Jennifer Major","orcid":"0000-0003-3140-5046","position":6,"is_corresponding":false},{"id":556388,"name":"Gary J. Brouhard","orcid":"0000-0001-9101-1247","position":7,"is_corresponding":false},{"id":1116737,"name":"Larissa Gheber","orcid":null,"position":8,"is_corresponding":false},{"id":326792,"name":"Steven S. Rosenfeld","orcid":"0000-0003-1909-7116","position":9,"is_corresponding":false},{"id":326793,"name":"Scott Forth","orcid":"0000-0002-2630-8017","position":10,"is_corresponding":false},{"id":815249,"name":"Adam G. Hendricks","orcid":"0000-0003-3635-1577","position":11,"is_corresponding":false},{"id":326794,"name":"Jawdat Al‐Bassam","orcid":"0000-0001-6625-2102","position":12,"is_corresponding":false},{"id":326784,"name":"Stanley Nithianantham","orcid":"0000-0001-6238-647X","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:14:23.749325Z","pmid":"37610838","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}