{"doi":"10.1091/mbc.e20-11-0723","title":"Spatial variation of microtubule depolymerization in large asters","abstract":"egg extract. We observed strong spatial regulation of depolymerization rates, which were higher in the aster interior compared with the periphery, and much less regulation of polymerization or catastrophe rates. We interpret these data in terms of a limiting component model, where aster growth results in lower levels of soluble tubulin and microtubule-associated proteins (MAPs) in the interior cytosol compared with that at the periphery. The steady-state polymer fraction of tubulin was ∼30%, so tubulin is not strongly depleted in the aster interior. We propose that the limiting component for microtubule assembly is a MAP that inhibits depolymerization, and that egg asters are tuned to low microtubule density.","journal":"Molecular Biology of the Cell","year":2021,"id":199675,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.95,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":776225,"name":"Franziska Decker","orcid":"0000-0002-3241-6575","position":1,"is_corresponding":false},{"id":776226,"name":"Paulo Caldas","orcid":"0000-0001-6730-4461","position":2,"is_corresponding":false},{"id":564734,"name":"James F. Pelletier","orcid":"0000-0001-8064-0259","position":3,"is_corresponding":false},{"id":776227,"name":"Martin Loose","orcid":"0000-0001-7309-9724","position":4,"is_corresponding":false},{"id":485192,"name":"Jan Brugués","orcid":"0000-0002-6731-4130","position":5,"is_corresponding":false},{"id":362447,"name":"Timothy J. Mitchison","orcid":"0000-0001-7781-1897","position":6,"is_corresponding":false},{"id":776224,"name":"Keisuke Ishihara","orcid":"0000-0002-8481-8680","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-18T23:50:40.558460Z","pmid":"33439671","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}