{"doi":"10.1091/mbc.e19-10-0568","title":"Spatial proximity of proteins surrounding zyxin under force-bearing conditions","abstract":"Sensing physical forces is a critical first step in mechano-transduction of cells. Zyxin, a LIM domain-containing protein, is recruited to force-bearing actin filaments and is thought to repair and strengthen them. Yet, the precise force-induced protein interactions surrounding zyxin remain unclear. Using BioID analysis, we identified proximal proteins surrounding zyxin under normal and force-bearing conditions by label-free mass spectrometry analysis. Under force-bearing conditions, increased biotinylation of α-actinin 1, α-actinin 4, and AFAP1 were detected, and these proteins accumulated along force-bearing actin fibers independently from zyxin, albeit at a lower intensity than zyxin. VASP also accumulated along force-bearing actin fibers in a zyxin-dependent manner, but the biotinylation of VASP remained constant regardless of force, supporting the model of a free zyxin-VASP complex in the cytoplasm being corecruited to tensed actin fibers. In addition, ARHGAP42, a RhoA GAP, was also identified as a proximal protein of zyxin and colocalized with zyxin along contractile actin bundles. The overexpression of ARHGAP42 reduced the rate of small wound closure, a zyxin-dependent process. These results demonstrate that the application of proximal biotinylation can resolve the proximity and composition of protein complexes as a function of force, which had not been possible with traditional biochemical analysis.","journal":"Molecular Biology of the Cell","year":2021,"id":189112,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9606,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":751108,"name":"Kyle A. Jacobs","orcid":"0000-0002-4411-7855","position":1,"is_corresponding":false},{"id":751721,"name":"Tzu Wei Lai","orcid":null,"position":2,"is_corresponding":false},{"id":751722,"name":"Reca Caballelo","orcid":null,"position":3,"is_corresponding":false},{"id":751109,"name":"Jacqueline L. Yee","orcid":"0000-0001-7860-2237","position":4,"is_corresponding":false},{"id":751110,"name":"Shuji Ueda","orcid":"0000-0002-5702-4471","position":5,"is_corresponding":false},{"id":599631,"name":"Volkmar Heinrich","orcid":"0000-0002-4932-5012","position":6,"is_corresponding":false},{"id":560332,"name":"Soichiro Yamada","orcid":"0000-0001-6413-0814","position":7,"is_corresponding":false},{"id":560330,"name":"Joleen S. Cheah","orcid":"0000-0002-1848-9583","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:49:14.547082Z","pmid":"33909446","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}