{"doi":"10.1091/mbc.e19-04-0209","title":"The\n                    <i>Drosophila</i>\n                    Afadin and ZO-1 homologues Canoe and Polychaetoid act in parallel to maintain epithelial integrity when challenged by adherens junction remodeling","abstract":"<jats:p>During morphogenesis, cells must change shape and move without disrupting tissue integrity. This requires cell–cell junctions to allow dynamic remodeling while resisting forces generated by the actomyosin cytoskeleton. Multiple proteins play roles in junctional–cytoskeletal linkage, but the mechanisms by which they act remain unclear. Drosophila Canoe maintains adherens junction–cytoskeletal linkage during gastrulation. Canoe’s mammalian homologue Afadin plays similar roles in cultured cells, working in parallel with ZO-1 proteins, particularly at multicellular junctions. We take these insights back to the fly embryo, exploring how cells maintain epithelial integrity when challenged by adherens junction remodeling during germband extension and dorsal closure. We found that Canoe helps cells maintain junctional–cytoskeletal linkage when challenged by the junctional remodeling inherent in mitosis, cell intercalation, and neuroblast invagination or by forces generated by the actomyosin cable at the leading edge. However, even in the absence of Canoe, many cells retain epithelial integrity. This is explained by a parallel role played by the ZO-1 homologue Polychaetoid. In embryos lacking both Canoe and Polychaetoid, cell junctions fail early, with multicellular junctions especially sensitive, leading to widespread loss of epithelial integrity. Our data suggest that Canoe and Polychaetoid stabilize Bazooka/Par3 at cell–cell junctions, helping maintain balanced apical contractility and tissue integrity.</jats:p>","journal":"Molecular Biology of the Cell","year":2019,"id":43349,"datarank":1.6968167738171362,"base_score":4.204692619390966,"endowment":4.204692619390966,"self_citation_contribution":0.6307038929086449,"citation_network_contribution":1.0661128809084912,"self_endowment_contribution":0.6307038929086449,"citer_contribution":1.0661128809084912,"corpus_percentile":null,"corpus_rank":null,"citation_count":66,"citer_count":48,"citers_with_citation_signal":39,"citers_with_endowment":39,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":206132,"name":"Kia Z. Perez-Vale","orcid":null,"position":1,"is_corresponding":false},{"id":206133,"name":"Kristina N. Schaefer","orcid":null,"position":2,"is_corresponding":false},{"id":206134,"name":"Mycah T. Sewell","orcid":null,"position":3,"is_corresponding":false},{"id":203139,"name":"Mark Peifer","orcid":null,"position":4,"is_corresponding":false},{"id":206131,"name":"Lathiena A. Manning","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.204692619390966,"endowment":4.204692619390966,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31188739","pmcid":"PMC6727765","openalex_id":"https://openalex.org/W2952815963","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM007092","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"P30 NS045892","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"K12 GM000678","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R35 GM118096","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R25 GM089569","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"F31 GM131521","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R25 GM055336","title":null},{"funder_name":"National Institutes of Health","grant_id":"2R25GM055336-04","title":"UNC CH MINORITY STUDENT DEVELOPMENT PROGRAM"},{"funder_name":"National Institutes of Health","grant_id":"5T32GM007092-28","title":"NRSA IN GENETICS"},{"funder_name":"National Institutes of Health","grant_id":"2K12GM000678-06","title":"Seeding Postdoctoral Innovators in Research &Education"},{"funder_name":"National Institutes of Health","grant_id":"5R35GM118096-04","title":"Regulating cell fate and shaping the body plan during morphogenesis and their alteration during oncogenesis"},{"funder_name":"National Institutes of Health","grant_id":"5R25GM089569-02","title":"UNC PREP in Biological & Biomedical Sciences"},{"funder_name":"National Institutes of Health","grant_id":"5R35GM118096-09","title":"Regulating cell fate and shaping the body plan during morphogenesis and their alteration during oncogenesis"},{"funder_name":"National Institutes of Health","grant_id":"5K12GM000678-27","title":"Institutional Research and Academic Career Development Awards (IRACDA) - 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