{"doi":"10.1091/mbc.e18-10-0651","title":"A method of quantifying centrosomes at the single-cell level in human normal and cancer tissue","abstract":"<jats:p> Centrosome abnormalities are emerging hallmarks of cancer. The overproduction of centrosomes (known as centrosome amplification) has been reported in a variety of cancers and is currently being explored as a promising target for therapy. However, to understand different types of centrosome abnormalities and their impact on centrosome function during tumor progression, as well as to identify tumor subtypes that would respond to the targeting of a centrosome abnormality, a reliable method for accurately quantifying centrosomes in human tissue samples is needed. Here, we established a method of quantifying centrosomes at a single-cell level in different types of human tissue samples. We tested multiple anti-centriole and pericentriolar-material antibodies to identify bona fide centrosomes and multiplexed these with cell border markers to identify individual cells within the tissue. High-resolution microscopy was used to generate multiple Z-section images, allowing us to acquire whole cell volumes in which to scan for centrosomes. The normal cells within the tissue serve as internal positive controls. Our method provides a simple, accurate way to distinguish alterations in centrosome numbers at the level of single cells. </jats:p>","journal":"Molecular Biology of the Cell","year":2019,"id":43379,"datarank":0.7506959050353001,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.3348075966993329,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.3348075966993329,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":11,"citers_with_citation_signal":9,"citers_with_endowment":9,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":61.25,"fair_percentile":92.70008795074759,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":206180,"name":"Beatrice S. Knudsen","orcid":null,"position":1,"is_corresponding":false},{"id":206181,"name":"Raymond B. Nagle","orcid":null,"position":2,"is_corresponding":false},{"id":205636,"name":"Gregory C. Rogers","orcid":"0000-0003-1870-1713","position":3,"is_corresponding":false},{"id":206182,"name":"Anne E. Cress","orcid":null,"position":4,"is_corresponding":false},{"id":52644,"name":"Mengdie Wang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.772588722239781,"endowment":2.772588722239781,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30699045","pmcid":"PMC6589791","openalex_id":"https://openalex.org/W2914745707","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM126035","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA242226","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM110166","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA159406","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA023074","title":null}],"total_grants":5,"fwci":1.1326,"citation_percentile":0.73994928,"influential_citations":0,"citation_trend":[{"year":2019,"count":2},{"year":2020,"count":3},{"year":2021,"count":1},{"year":2022,"count":4},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":3}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6589791","host_type":"repository"},{"url":"https://doi.org/10.1091/mbc.e18-10-0651","host_type":"HYBRID"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6589791","host_type":"repository"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30699045","host_type":"repository"},{"url":"http://hdl.handle.net/10150/633342","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC6589791","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC6589791?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Microtubule and mitosis dynamics","Cancer Cells and Metastasis","Cellular Mechanics and Interactions","Biology","Medicine","Carrier Proteins","Centrioles","Centrosome","Humans","Neoplasms","Single-Cell Analysis","Tubulin"],"mesh_terms":["Carrier Proteins","Centrioles","Humans","Neoplasms","Tubulin","Centrosome","Single-Cell Analysis"],"keywords":["Centrosome","Biology","Centriole","Centrosome cycle","Cell biology","Microtubule","Cell","Genetics","Cell cycle"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and 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