{"doi":"10.1091/mbc.e18-06-0339","title":"Cargo induces retromer-mediated membrane remodeling on membranes","abstract":"<jats:p> Endosomes serve as a central sorting station of lipids and proteins that arrive via vesicular carrier from the plasma membrane and the Golgi complex. At the endosome, retromer complexes sort selected receptors and membrane proteins into tubules or vesicles that bud off the endosome. The mature endosome finally fuses with the lysosome. Retromer complexes consist of a cargo selection complex (CSC) and a membrane remodeling part (sorting nexin [SNX]-Bin/amphiphysin/Rvs [BAR], or Snx3 in yeast) and different assemblies of retromer mediate recycling of different cargoes. Due to this complexity, the exact order of events that results in carrier formation is not yet understood. Here, we reconstituted this process on giant unilamellar vesicles together with purified retromer complexes from yeast and selected cargoes. Our data reveal that the membrane remodeling activity of both Snx3 and the SNX-BAR complex is strongly reduced at low concentrations, which can be reactivated by CSC. At even lower concentrations, these complexes still associate with membranes, but only remodel membranes in the presence of their specific cargoes. Our data thus favor a simple model, where cargo functions as a specific trigger of retromer-mediated sorting on endosomes. </jats:p>","journal":"Molecular Biology of the Cell","year":2018,"id":24249,"datarank":1.3569301246840553,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"self_citation_contribution":0.5416376868966337,"citation_network_contribution":0.8152924377874214,"self_endowment_contribution":0.5416376868966337,"citer_contribution":0.8152924377874214,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":35,"citers_with_citation_signal":28,"citers_with_endowment":28,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":146146,"name":"Christian Ungermann","orcid":null,"position":1,"is_corresponding":false},{"id":146145,"name":"Latha Kallur Purushothaman","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30188774","pmcid":"PMC6249844","openalex_id":"https://openalex.org/W2891130903","authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"180879236/SFB 944","title":"Physiology and Dynamics of Cellular Microcompartments"}],"total_grants":1,"fwci":1.764,"citation_percentile":0.83379596,"influential_citations":2,"citation_trend":[{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":6},{"year":2021,"count":6},{"year":2022,"count":4},{"year":2023,"count":2},{"year":2024,"count":6},{"year":2025,"count":5},{"year":2026,"count":4}],"oa_status":"closed","license":"CC BY NC SA","oa_locations":[{"url":"https://doi.org/10.1091/mbc.e18-06-0339","host_type":"HYBRID"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30188774","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6249844","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC6249844","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC6249844?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1091/mbc.E18-06-0339","host_type":""},{"url":"https://dx.doi.org/10.1091/mbc.e18-06-0339","host_type":""}],"fields_of_study":["Cellular transport and secretion","Lipid Membrane Structure and Behavior","Endoplasmic Reticulum Stress and Disease","Medicine","Biology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Cell Membrane","Multiprotein Complexes","Nerve Tissue Proteins","Protein Transport","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Sorting Nexins"],"mesh_terms":["Cell Membrane","Nerve Tissue Proteins","Saccharomyces cerevisiae","Protein Transport","Saccharomyces cerevisiae Proteins","Multiprotein Complexes","Sorting Nexins"],"keywords":["Retromer","Endosome","Sorting nexin","Cell biology","Vesicular Transport Proteins","Amphiphysin","Biology","Vesicle","Lysosome","Golgi apparatus","Membrane","Endocytosis","Vacuolar protein sorting","Biochemistry","Receptor","Intracellular","Endoplasmic reticulum","Dynamin","Protein Transport","Saccharomyces cerevisiae Proteins","Multiprotein Complexes","Cell Membrane","Nerve Tissue Proteins","Articles","Saccharomyces cerevisiae","Sorting Nexins"],"sdg_mappings":[{"sdg_number":12,"sdg_label":"12. 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