{"doi":"10.1091/mbc.e13-12-0749","title":"Capping protein is essential for cell migration in vivo and for filopodial morphology and dynamics","abstract":"<jats:p>Capping protein (CP) binds to barbed ends of growing actin filaments and inhibits elongation. CP is essential for actin-based motility in cell-free systems and in Dictyostelium. Even though CP is believed to be critical for creating the lamellipodial actin structure necessary for protrusion and migration, CP's role in mammalian cell migration has not been directly tested. Moreover, recent studies have suggested that structures besides lamellipodia, including lamella and filopodia, may have unappreciated roles in cell migration. CP has been postulated to be absent from filopodia, and thus its role in filopodial activity has remained unexplored. We report that silencing CP in both cultured mammalian B16F10 cells and in neurons of developing neocortex impaired cell migration. Moreover, we unexpectedly observed that low levels of CP were detectable in the majority of filopodia. CP depletion decreased filopodial length, altered filopodial shape, and reduced filopodial dynamics. Our results support an expansion of the potential roles that CP plays in cell motility by implicating CP in filopodia as well as in lamellipodia, both of which are important for locomotion in many types of migrating cells.</jats:p>","journal":"Molecular Biology of the Cell","year":2014,"id":663568,"datarank":0.611630616585858,"base_score":4.07753744390572,"endowment":4.07753744390572,"self_citation_contribution":0.611630616585858,"citation_network_contribution":0.0,"self_endowment_contribution":0.611630616585858,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":58,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":575034,"name":"Susumu Antoku","orcid":"0000-0002-0698-6224","position":1,"is_corresponding":false},{"id":1732506,"name":"Jean-Michel Saffin","orcid":null,"position":2,"is_corresponding":false},{"id":1732507,"name":"Jon A. 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Moreover, recent studies have suggested that structures besides lamellipodia, including lamella and filopodia, may have unappreciated roles in cell migration. CP has been postulated to be absent from filopodia, and thus its role in filopodial activity has remained unexplored. We report that silencing CP in both cultured mammalian B16F10 cells and in neurons of developing neocortex impaired cell migration. Moreover, we unexpectedly observed that low levels of CP were detectable in the majority of filopodia. CP depletion decreased filopodial length, altered filopodial shape, and reduced filopodial dynamics. Our results support an expansion of the potential roles that CP plays in cell motility by implicating CP in filopodia as well as in lamellipodia, both of which are important for locomotion in many types of migrating cells.</jats:p>","is_dataset_classified":null,"base_score":4.060443010546419,"endowment":4.060443010546419,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24829386","pmcid":"PMC4091828","openalex_id":"https://openalex.org/W2160370078","authors":[],"funders":[{"funder_name":"NIMH NIH HHS","grant_id":"MH087823","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA015704","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"NS080194","title":null},{"funder_name":"NIMH NIH HHS","grant_id":"R01 MH087823","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"R01 NS080194","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01NS080194-30","title":"Regulation of cortical neuron migration"},{"funder_name":"National Institutes of Health","grant_id":"5R01MH087823-02","title":"Mechanisms of Dendritic Spine Stability"}],"total_grants":7,"fwci":3.234,"citation_percentile":0.92287283,"influential_citations":0,"citation_trend":[{"year":2014,"count":2},{"year":2015,"count":11},{"year":2016,"count":6},{"year":2017,"count":4},{"year":2018,"count":4},{"year":2019,"count":6},{"year":2020,"count":6},{"year":2021,"count":5},{"year":2022,"count":3},{"year":2023,"count":5},{"year":2024,"count":2},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"green","license":"CC BY NC SA","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4091828","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4091828","host_type":"repository"},{"url":"https://doi.org/10.1091/mbc.e13-12-0749","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24829386","host_type":"repository"},{"url":"https://escholarship.org/uc/item/00s4c1hs","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4091828","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4091828?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1091/mbc.E13-12-0749","host_type":""},{"url":"https://dx.doi.org/10.1091/mbc.e13-12-0749","host_type":""},{"url":"https://escholarship.org/content/qt00s4c1hs/qt00s4c1hs.pdf","host_type":""},{"url":"https://doi.org/https://doi.org/10.1091/mbc.E13-12-0749","host_type":""}],"fields_of_study":["Cellular Mechanics and Interactions","Advanced Fluorescence Microscopy Techniques","Force Microscopy Techniques and Applications","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Actins","Animals","Cell Movement","Pseudopodia","Cell Line, Tumor","Cell Shape","CapZ Actin Capping Protein","Mice","Gene Knockdown Techniques"],"keywords":["Filopodia","Lamellipodium","Biology","Pseudopodia","Cell biology","Cell migration","Motility","Dictyostelium","Actin","Cell","Biochemistry","CapZ Actin Capping Protein","570","Tumor","Articles","Actins","Cell Line","Mice","Cell Movement","Cell Line, Tumor","Gene Knockdown Techniques","616","Animals","Cell Shape"],"sdg_mappings":[{"sdg_number":1,"sdg_label":"1. 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