{"doi":"10.1091/mbc.e13-05-0229","title":"Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences","abstract":"<jats:p>The establishment and maintenance of higher-order structure at centromeres is essential for accurate chromosome segregation. The monopolin complex is thought to cross-link multiple kinetochore complexes to prevent merotelic attachments that result in chromosome missegregation. This model is based on structural analysis and the requirement that monopolin execute mitotic and meiotic chromosome segregation in Schizosaccharomyces pombe, which has more than one kinetochore–microtubule attachment/centromere, and co-orient sister chromatids in meiosis I in Saccharomyces cerevisiae. Recent data from S. pombe suggest an alternative possibility: that the recruitment of condensin is the primary function of monopolin. Here we test these models using the yeast Candida albicans. C. albicans cells lacking monopolin exhibit defects in chromosome segregation, increased distance between centromeres, and decreased stability of several types of repeat DNA. Of note, changing kinetochore–microtubule copy number from one to more than one kinetochore–microtubule/centromere does not alter the requirement for monopolin. Furthermore, monopolin recruits condensin to C. albicans centromeres, and overexpression of condensin suppresses chromosome segregation defects in strains lacking monopolin. We propose that the key function of monopolin is to recruit condensin in order to promote the assembly of higher-order structure at centromere and repetitive DNA.</jats:p>","journal":"Molecular Biology of the Cell","year":2013,"id":655968,"datarank":0.4943755299006494,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.0,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1712326,"name":"Shelly E. Applen Clancey","orcid":null,"position":1,"is_corresponding":false},{"id":315180,"name":"Jeremy M. Chacón","orcid":"0000-0002-6129-2604","position":2,"is_corresponding":false},{"id":523860,"name":"Melissa K. Gardner","orcid":"0000-0001-5906-7363","position":3,"is_corresponding":false},{"id":21604,"name":"Judith Berman","orcid":"0000-0002-8577-0084","position":4,"is_corresponding":false},{"id":854348,"name":"Laura S. Burrack","orcid":"0000-0002-0821-0980","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences","abstract":"<jats:p>The establishment and maintenance of higher-order structure at centromeres is essential for accurate chromosome segregation. The monopolin complex is thought to cross-link multiple kinetochore complexes to prevent merotelic attachments that result in chromosome missegregation. This model is based on structural analysis and the requirement that monopolin execute mitotic and meiotic chromosome segregation in Schizosaccharomyces pombe, which has more than one kinetochore–microtubule attachment/centromere, and co-orient sister chromatids in meiosis I in Saccharomyces cerevisiae. Recent data from S. pombe suggest an alternative possibility: that the recruitment of condensin is the primary function of monopolin. Here we test these models using the yeast Candida albicans. C. albicans cells lacking monopolin exhibit defects in chromosome segregation, increased distance between centromeres, and decreased stability of several types of repeat DNA. Of note, changing kinetochore–microtubule copy number from one to more than one kinetochore–microtubule/centromere does not alter the requirement for monopolin. Furthermore, monopolin recruits condensin to C. albicans centromeres, and overexpression of condensin suppresses chromosome segregation defects in strains lacking monopolin. We propose that the key function of monopolin is to recruit condensin in order to promote the assembly of higher-order structure at centromere and repetitive DNA.</jats:p>","is_dataset_classified":null,"base_score":3.295836866004329,"endowment":3.295836866004329,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23885115","pmcid":"PMC3771944","openalex_id":"https://openalex.org/W2169777858","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI075096","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R21 GM100122","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM100122","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI075096","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM103833","title":null},{"funder_name":"National Institutes of Health","grant_id":"3R01AI075096-05S1","title":"Centromere Structure and Function in Candida albicans"},{"funder_name":"National Institutes of Health","grant_id":"5R21GM100122-02","title":"Computational Modeling and Analysis of Kinetochore Dynamics during Mitosis"}],"total_grants":7,"fwci":3.0005,"citation_percentile":0.91222959,"influential_citations":0,"citation_trend":[{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":4},{"year":2017,"count":1},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":4},{"year":2021,"count":2},{"year":2022,"count":2},{"year":2024,"count":3},{"year":2025,"count":1}],"oa_status":"closed","license":"CC BY NC SA","oa_locations":[{"url":"https://doi.org/10.1091/mbc.e13-05-0229","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23885115","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3771944","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3771944","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3771944?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1091/mbc.E13-05-0229","host_type":""},{"url":"https://dx.doi.org/10.1091/mbc.e13-05-0229","host_type":""}],"fields_of_study":["Chromosomal and Genetic Variations","Fungal and yeast genetics research","Microtubule and mitosis dynamics","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Adenosine Triphosphatases","Candida albicans","Cell Cycle","Centromere","DNA-Binding Proteins","DNA, Fungal","DNA, Ribosomal","Fungal Proteins","Metaphase","Microtubules","Spindle Apparatus","Models, Biological","Repetitive Sequences, Nucleic Acid","Chromosomes, Fungal","Genome, Fungal","Gene Deletion","Kinetochores","Chromosome Segregation","Protein Transport","Multiprotein Complexes"],"keywords":["Centromere","Condensin","Kinetochore","Biology","Chromosome segregation","Sister chromatids","Genetics","Chromosome","Mitosis","Chromatid","Cell biology","Schizosaccharomyces pombe","Cohesin","Saccharomyces cerevisiae","Yeast","Gene","Adenosine Triphosphatases","Cell Cycle","Articles","Spindle Apparatus","DNA, Ribosomal","Microtubules","Models, Biological","DNA-Binding Proteins","Fungal Proteins","Protein Transport","Multiprotein Complexes","Candida albicans","Chromosomes, Fungal","Genome, Fungal","DNA, Fungal","Kinetochores","Gene Deletion","Metaphase","Repetitive Sequences, Nucleic Acid"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T19:29:28.916466Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}