{"doi":"10.1091/mbc.e13-03-0149","title":"Abnormal centrosomal structure and duplication in Cep135-deficient vertebrate cells","abstract":"<jats:p>Centrosomes are key microtubule-organizing centers that contain a pair of centrioles, conserved cylindrical, microtubule-based structures. Centrosome duplication occurs once per cell cycle and relies on templated centriole assembly. In many animal cells this process starts with the formation of a radially symmetrical cartwheel structure. The centrosomal protein Cep135 localizes to this cartwheel, but its role in vertebrates is not well understood. Here we examine the involvement of Cep135 in centriole function by disrupting the Cep135 gene in the DT40 chicken B-cell line. DT40 cells that lack Cep135 are viable and show no major defects in centrosome composition or function, although we note a small decrease in centriole numbers and a concomitant increase in the frequency of monopolar spindles. Furthermore, electron microscopy reveals an atypical structure in the lumen of Cep135-deficient centrioles. Centrosome amplification after hydroxyurea treatment increases significantly in Cep135-deficient cells, suggesting an inhibitory role for the protein in centrosome reduplication during S-phase delay. We propose that Cep135 is required for the structural integrity of centrioles in proliferating vertebrate cells, a role that also limits centrosome amplification in S-phase–arrested cells.</jats:p>","journal":"Molecular Biology of the Cell","year":2013,"id":42310,"datarank":1.423359935469259,"base_score":3.332204510175204,"endowment":3.332204510175204,"self_citation_contribution":0.49983067652628066,"citation_network_contribution":0.9235292589429782,"self_endowment_contribution":0.49983067652628066,"citer_contribution":0.9235292589429782,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":26,"citers_with_citation_signal":24,"citers_with_endowment":24,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":202685,"name":"Monika Pütz","orcid":null,"position":1,"is_corresponding":false},{"id":202686,"name":"Pierce Lalor","orcid":null,"position":2,"is_corresponding":false},{"id":202687,"name":"Peter Dockery","orcid":null,"position":3,"is_corresponding":false},{"id":202688,"name":"Ryoko Kuriyama","orcid":null,"position":4,"is_corresponding":false},{"id":202689,"name":"Fanni Gergely","orcid":null,"position":5,"is_corresponding":false},{"id":202690,"name":"Ciaran G. Morrison","orcid":null,"position":6,"is_corresponding":false},{"id":202684,"name":"Burcu Inanç","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.332204510175204,"endowment":3.332204510175204,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23864714","pmcid":"PMC3756917","openalex_id":"https://openalex.org/W1995931074","authors":[],"funders":[{"funder_name":"Cancer Research UK","grant_id":"17043","title":null},{"funder_name":"Science Foundation Ireland","grant_id":"08/IN.1/B1029","title":"Mechanisms and cellular consequences of centrosome amplification"},{"funder_name":"Science Foundation Ireland","grant_id":"10/IN.1/B2972","title":"Centrosomal control of the cell cycle"}],"total_grants":3,"fwci":1.6399,"citation_percentile":0.81040967,"influential_citations":3,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":3},{"year":2015,"count":5},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":3},{"year":2020,"count":3},{"year":2021,"count":2},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"CC BY NC ND","oa_locations":[{"url":"https://doi.org/10.1091/mbc.e13-03-0149","host_type":"HYBRID"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23864714","host_type":"repository"},{"url":"http://hdl.handle.net/10379/12037","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3756917","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3756917","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3756917?pdf=render","host_type":"Europe_PMC"},{"url":"https://dx.doi.org/10.13025/29016","host_type":""},{"url":"http://dx.doi.org/10.1091/mbc.E13-03-0149","host_type":""},{"url":"https://dx.doi.org/10.1091/mbc.e13-03-0149","host_type":""},{"url":"https://doi.org/10.13025/29016","host_type":""},{"url":"https://hdl.handle.net/10379/12037","host_type":""}],"fields_of_study":["Microtubule and mitosis dynamics","Photosynthetic Processes and Mechanisms","Protist diversity and phylogeny","Biology","Medicine","0301 basic medicine","03 medical and health sciences","0303 health sciences","Animals","Avian Proteins","Carrier Proteins","Cell Cycle","Cell Division","Cell Line","Cell Proliferation","Cell Survival","Centrioles","Centrosome","Chickens","Gene Knock-In Techniques","Gene Knockout Techniques","Gene Targeting","Microtubules","Mitosis","S Phase"],"mesh_terms":["Animals","Carrier Proteins","Cell Cycle","Cell Division","Cell Line","Cell Survival","Centrioles","Chickens","Microtubules","Mitosis","S Phase","Centrosome","Gene Targeting","Avian Proteins","Cell Proliferation","Gene Knockout Techniques","Gene Knock-In Techniques"],"keywords":["Centrosome","Centriole","Biology","Centrosome cycle","Cell biology","Basal body","Mitosis","Microtubule","Spindle pole body","Cell division","Cell cycle","Cell","Genetics","Flagellum","Spindle apparatus","Gene","Cell Survival","mammalian-cells","cep135","amplification","Microtubules","Cell Line","S Phase","Avian Proteins","Gene Knockout Techniques","Animals","Gene Knock-In Techniques","9-fold symmetry","Cell Proliferation","Centrioles","cartwheel","Externally hosted open access publications with University of Galway authors","microtubule organization","DNA-damage response","Articles","Gene Targeting","Carrier Proteins","protein","Chickens"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T02:16:35.468268Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}