{"doi":"10.1091/mbc.e07-12-1259","title":"Spliceosomal Small Nuclear Ribonucleoprotein Particles Repeatedly Cycle through Cajal Bodies","abstract":"<jats:p>The Cajal body (CB) is a nuclear structure closely associated with import and biogenesis of small nuclear ribonucleoprotein particles (snRNPs). Here, we tested whether CBs also contain mature snRNPs and whether CB integrity depends on the ongoing snRNP splicing cycle. Sm proteins tagged with photoactivatable and color-maturing variants of fluorescent proteins were used to monitor snRNP behavior in living cells over time; mature snRNPs accumulated in CBs, traveled from one CB to another, and they were not preferentially replaced by newly imported snRNPs. To test whether CB integrity depends on the snRNP splicing cycle, two human orthologues of yeast proteins involved in distinct steps in spliceosome disassembly after splicing, hPrp22 and hNtr1, were depleted by small interfering RNA treatment. Surprisingly, depletion of either protein led to the accumulation of U4/U6 snRNPs in CBs, suggesting that reassembly of the U4/U6·U5 tri-snRNP was delayed. Accordingly, a relative decrease in U5 snRNPs compared with U4/U6 snRNPs was observed in CBs, as well as in nuclear extracts of treated cells. Together, the data show that particular phases of the spliceosome cycle are compartmentalized in living cells, with reassembly of the tri-snRNP occurring in CBs.</jats:p>","journal":"Molecular Biology of the Cell","year":2008,"id":35692,"datarank":3.1552600162185023,"base_score":4.418840607796598,"endowment":4.418840607796598,"self_citation_contribution":0.6628260911694899,"citation_network_contribution":2.492433925049012,"self_endowment_contribution":0.6628260911694899,"citer_contribution":2.492433925049012,"corpus_percentile":null,"corpus_rank":null,"citation_count":82,"citer_count":67,"citers_with_citation_signal":59,"citers_with_endowment":59,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":182304,"name":"Jarmila Přidalová-Hnilicová","orcid":null,"position":1,"is_corresponding":false},{"id":178586,"name":"Ivan Novotný","orcid":null,"position":2,"is_corresponding":false},{"id":182305,"name":"Martina Huranová","orcid":null,"position":3,"is_corresponding":false},{"id":182306,"name":"Michaela Blažíková","orcid":null,"position":4,"is_corresponding":false},{"id":182307,"name":"Xin Wen","orcid":null,"position":5,"is_corresponding":false},{"id":182308,"name":"Aparna K. Sapra","orcid":null,"position":6,"is_corresponding":false},{"id":178582,"name":"Karla M. Neugebauer","orcid":null,"position":7,"is_corresponding":false},{"id":178600,"name":"David Staněk","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.418840607796598,"endowment":4.418840607796598,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18367544","pmcid":"PMC2397305","openalex_id":"https://openalex.org/W2102152121","authors":[],"funders":[],"total_grants":0,"fwci":4.211,"citation_percentile":0.94769976,"influential_citations":5,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":1},{"year":2014,"count":8},{"year":2015,"count":6},{"year":2016,"count":5},{"year":2017,"count":5},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":1},{"year":2021,"count":3},{"year":2022,"count":5},{"year":2023,"count":6},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2397305","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc2397305?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2397305","host_type":"repository"},{"url":"https://doi.org/10.1091/mbc.e07-12-1259","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18367544","host_type":"repository"},{"url":"http://edoc.mpg.de/414332","host_type":"repository"},{"url":"http://hdl.handle.net/21.11116/0000-0001-0E9B-2","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","Neurogenetic and Muscular Disorders Research","Nuclear Structure and Function","Medicine","Biology","Biomarkers","Carrier Proteins","Coiled Bodies","HeLa Cells","Humans","RNA, Small Interfering","RNA-Binding Proteins","Ribonucleoprotein, U4-U6 Small Nuclear","Ribonucleoprotein, U5 Small Nuclear","Ribonucleoproteins, Small Nuclear","Spliceosomes","Survival of Motor Neuron 1 Protein"],"mesh_terms":["Carrier Proteins","HeLa Cells","Humans","Biomarkers","RNA-Binding Proteins","Spliceosomes","Ribonucleoproteins, Small Nuclear","Ribonucleoprotein, U4-U6 Small Nuclear","Ribonucleoprotein, U5 Small Nuclear","Coiled Bodies","RNA, Small Interfering","Survival of Motor Neuron 1 Protein","Hela Cells"],"keywords":["snRNP","Cajal body","Biology","Small nuclear ribonucleoprotein","Spliceosome","RNA splicing","Ribonucleoprotein","Cell biology","Small nuclear RNA","RNA","Genetics","Gene","Non-coding RNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T07:32:11.619385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}